Ubiquitin-like modifier-activating enzyme 6 (Ubiquitin-activating enzyme 6) (EC 6.2.1.45) (Monocyte protein 4) (MOP-4) (Ubiquitin-activating enzyme E1-like protein 2) (E1-L2)
1_MEGSE 6_ PVAAH 11_ QGEEA 16_ SCSSW 21_ GTGST 26_ NKNLP 31_ IMSTA 36_ SVEID 41_ DALYS 46_ RQRYV 51_ LGDTA 56_ MQKMA 61_ KSHVF 66_ LSGMG 71_ GLGLE 76_ IAKNL 81_ VLAGI 86_ KAVTI 91_ HDTEK 96_ CQAWD 101_ LGTNF 106_ FLSED 111_ DVVNK 116_ RNRAE 121_ AVLKH 126_ IAELN 131_ PYVHV 136_ TSSSV 141_ PFNET 146_ TDLSF 151_ LDKYQ 156_ CVVLT 161_ EMKLP 166_ LQKKI 171_ NDFCR 176_ SQCPP 181_ IKFIS 186_ ADVHG 191_ IWSRL 196_ FCDFG 201_ DEFEV 206_ LDTTG 211_ EEPKE 216_ IFISN 221_ ITQAN 226_ PGIVT 231_ CLENH 236_ PHKLE 241_ TGQFL 246_ TFREI 251_ NGMTG 256_ LNGSI 261_ QQITV 266_ ISPFS 271_ FSIGD 276_ TTELE 281_ PYLHG 286_ GIAVQ 291_ VKTPK 296_ TVFFE 301_ SLERQ 306_ LKHPK 311_ CLIVD 316_ FSNPE 321_ APLEI 326_ HTAML 331_ ALDQF 336_ QEKYS 341_ RKPNV 346_ GCQQD 351_ SEELL 356_ KLATS 361_ ISETL 366_ EEKPD 371_ VNADI 376_ VHWLS 381_ WTAQG 386_ FLSPL 391_ AAAVG 396_ GVASQ 401_ EVLKA 406_ VTGKF 411_ SPLCQ 416_ WLYLE 421_ AADIV 426_ ESLGK 431_ PECEE 436_ FLPRG 441_ DRYDA 446_ LRACI 451_ GDTLC 456_ QKLQN 461_ LNIFL 466_ VGCGA 471_ IGCEM 476_ LKNFA 481_ LLGVG 486_ TSKEK 491_ GMITV 496_ TDPDL 501_ IEKSN 506_ LNRQF 511_ LFRPH 516_ HIQKP 521_ KSYTA 526_ ADATL 531_ KINSQ 536_ IKIDA 541_ HLNKV 546_ CPTTE 551_ TIYND 556_ EFYTK 561_ QDVII 566_ TALDN 571_ VEARR 576_ YVDSR 581_ CLANL 586_ RPLLD 591_ SGTMG 596_ TKGHT 601_ EVIVP 606_ HLTES 611_ YNSHR 616_ DPPEE 621_ EIPFC 626_ TLKSF 631_ PAAIE 636_ HTIQW 641_ ARDKF 646_ ESSFS 651_ HKPSL 656_ FNKFW 661_ QTYSS 666_ AEEVL 671_ QKIQS 676_ GHSLE 681_ GCFQV 686_ IKLLS 691_ RRPRN 696_ WSQCV 701_ ELARL 706_ KFEKY 711_ FNHKA 716_ LQLLH 721_ CFPLD 726_ IRLKD 731_ GSLFW 736_ QSPKR 741_ PPSPI 746_ KFDLN 751_ EPLHL 756_ SFLQN 761_ AAKLY 766_ ATVYC 771_ IPFAE 776_ EDLSA 781_ DALLN 786_ ILSEV 791_ KIQEF 796_ KPSNK 801_ VVQTD 806_ ETARK 811_ PDHVP 816_ ISSED 821_ ERNAI 826_ FQLEK 831_ AILSN 836_ EATKS 841_ DLQMA 846_ VLSFE 851_ KDDDH 856_ NGHID 861_ FITAA 866_ SNLRA 871_ KMYSI 876_ EPADR 881_ FKTKR 886_ IAGKI 891_ IPAIA 896_ TTTAT 901_ VSGLV 906_ ALEMI 911_ KVTGG 916_ YPFEA 921_ YKNCF 926_ LNLAI 931_ PIVVF 936_ TETTE 941_ VRKTK 946_ IRNGI 951_ SFTIW 956_ DRWTV 961_ HGKED 966_ FTLLD 971_ FINAV 976_ KEKYG 981_ IEPTM 986_ VVQGV 991_ KMLYV 996_ PVMPG 1001_ HAKRL 1006_ KLTMH 1011_ KLVKP 1016_ TTEKK 1021_ YVDLT 1026_ VSFAP 1031_ DIDGD 1036_ EDLPG 1041_ PPVRY 1046_YFSHD
1: Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP (PubMed:35970836, PubMed:35986001). Specific for ubiquitin, does not activate ubiquitin-like peptides. Also activates UBD/FAT10 conjugation via adenylation of its C-terminal glycine (PubMed:17889673, PubMed:35970836, PubMed:35986001). Differs from UBE1 in its specificity for substrate E2 charging. Does not charge cell cycle E2s, such as CDC34. Essential for embryonic development. Isoform 2 may play a key role in ubiquitin system and may influence spermatogenesis and male fertility