tRNA wybutosine-synthesizing protein 5 (hTYW5) (EC 1.14.11.42) (tRNA(Phe) (7-(3-amino-3-carboxypropyl)wyosine(37)-C(2))-hydroxylase)
1_MAGQH 6_ LPVPR 11_ LEGVS 16_ REQFM 21_ QHLYP 26_ QRKPL 31_ VLEGI 36_ DLGPC 41_ TSKWT 46_ VDYLS 51_ QVGGK 56_ KEVKI 61_ HVAAV 66_ AQMDF 71_ ISKNF 76_ VYRTL 81_ PFDQL 86_ VQRAA 91_ EEKHK 96_ EFFVS 101_ EDEKY 106_ YLRSL 111_ GEDPR 116_ KDVAD 121_ IRKQF 126_ PLLKG 131_ DIKFP 136_ EFFKE 141_ EQFFS 146_ SVFRI 151_ SSPGL 156_ QLWTH 161_ YDVMD 166_ NLLIQ 171_ VTGKK 176_ RVVLF 181_ SPRDA 186_ QYLYL 191_ KGTKS 196_ EVLNI 201_ DNPDL 206_ AKYPL 211_ FSKAR 216_ RYECS 221_ LEAGD 226_ VLFIP 231_ ALWFH 236_ NVISE 241_ EFGVG 246_ VNIFW 251_ KHLPS 256_ ECYDK 261_ TDTYG 266_ NKDPT 271_ AASRA 276_ AQILD 281_ RALKT 286_ LAELP 291_ EEYRD 296_ FYARR 301_ MVLHI 306_QDKAY
1: tRNA hydroxylase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Catalyzes the hydroxylation of 7-(a-amino-a-carboxypropyl)wyosine (yW-72) into undermodified hydroxywybutosine (OHyW*). OHyW* being further transformed into hydroxywybutosine (OHyW) by LCMT2/TYW4. OHyW is a derivative of wybutosine found in higher eukaryotes