Protein monoglycylase TTLL8 (EC 6.3.2.-) (Tubulin--tyrosine ligase-like protein 8)
1_MEPER 6_ KGLSL 11_ ASSSD 16_ GDGRE 21_ ENKLK 26_ QGISQ 31_ DLASS 36_ SRLDR 41_ YKIAR 46_ QLTEK 51_ AIKEK 56_ KIFSI 61_ YGHYP 66_ VVRAA 71_ LRRKG 76_ WVEKK 81_ FHFLP 86_ KVIPD 91_ VEDEG 96_ ARVND 101_ DTCAK 106_ VKENQ 111_ EMALE 116_ KTDNI 121_ HDVMS 126_ RLVKN 131_ EMPYL 136_ LWTIK 141_ RDIID 146_ YHSLT 151_ YDQML 156_ NHYAK 161_ TASFT 166_ TKIGL 171_ CVNMR 176_ SLPWY 181_ VPANP 186_ DSFFP 191_ RCYSL 196_ CTESE 201_ QQEFL 206_ EDFRR 211_ TMASS 216_ ILKWV 221_ VSHQS 226_ CSRSS 231_ RSKPR 236_ DQREE 241_ AGSSD 246_ LSSRQ 251_ DAENA 256_ EAKLR 261_ GLPGQ 266_ LVDIA 271_ CKVCQ 276_ AYLGQ 281_ LEHED 286_ IDTSA 291_ DAVED 296_ LTEAE 301_ WEDLT 306_ QQYYS 311_ LVHGD 316_ AFISN 321_ SRNYF 326_ SQCQA 331_ LLNRI 336_ TSVNP 341_ QTDID 346_ GLRNI 351_ WIIKP 356_ AAKSR 361_ GRDIV 366_ CMDRV 371_ EEILE 376_ LAAAD 381_ HPLSR 386_ DNKWV 391_ VQKYI 396_ ETPLL 401_ ICDTK 406_ FDIRQ 411_ WFLVT 416_ DWNPL 421_ TIWFY 426_ KESYL 431_ RFSTQ 436_ RFSLD 441_ KLDSA 446_ IHLCN 451_ NAVQK 456_ YLKND 461_ VGRSP 466_ LLPAH 471_ NMWTS 476_ TRFQE 481_ YLQRQ 486_ GRGAV 491_ WGSVI 496_ YPSMK 501_ KAIAH 506_ AMKVA 511_ QDHVE 516_ PRKNS 521_ FELYG 526_ ADFVL 531_ GRDFR 536_ PWLIE 541_ INSSP 546_ TMHPS 551_ TPVTA 556_ QLCAQ 561_ VQEDT 566_ IKVAV 571_ DRSCD 576_ IGNFE 581_ LLWRQ 586_ PVVEP 591_ PPFSG 596_ SDLCV 601_ AGVSV 606_ RRARR 611_ QVLPV 616_ CNLKA 621_ SASLL 626_ DAQPL 631_ KARGP 636_ SAMPD 641_ PAQGP 646_ PSPAL 651_ QRDLG 656_ LKEEK 661_ GLPLA 666_ LLAPL 671_ RGAAE 676_ SGGAA 681_ QPTRT 686_ KAAGK 691_ VELPA 696_ CPCRH 701_ VDSQA 706_ PNTGV 711_ PVAQP 716_ AKSWD 721_ PNQLN 726_ AHPLE 731_ PVLRG 736_ LKTAE 741_ GALRP 746_ PPGGK 751_ GEGTV 756_ CSRLP 761_ HHGHH 766_ VAACQ 771_ TTGTT 776_ WDGGP 781_ GVCFL 786_ RQLLA 791_ SELPM 796_ GPGLP 801_ RDPRA 806_ PPCLV 811_ CRGLL 816_ PPAGP 821_ CKRCR 826_ SFCAA 831_ VLQGA 836_ SFVRL 841_GGRSC
1: Monoglycylase which modifies both tubulin and non-tubulin proteins, adding a single glycine to the gamma-carboxyl groups of specific glutamate residues to generate monoglycine side chains within the C-terminal tail of target proteins. Not involved in elongation step of the polyglycylation reaction. Preferentially monoglycylates alpha-tubulin over beta-tubulin. Together with TTLL3, mediates microtubule glycylation of primary and motile cilia, which is essential for their stability and maintenance. Together with TTLL3, glycylates sperm flagella which regulates axonemal dynein motor activity, thereby controlling flagellar beat, directional sperm swimming and male fertility. Monoglycylates non-tubulin proteins such as ANP32A, ANP32B, SET, NCL and NAP1