Nucleoside diphosphate kinase, mitochondrial (NDK) (NDP kinase, mitochondrial) (EC 2.7.4.6) (Nucleoside diphosphate kinase D) (NDPKD) (nm23-H4)
1_MGGLF 6_ WRSAL 11_ RGLRC 16_ GPRAP 21_ GPSLL 26_ VRHGS 31_ GGPSW 36_ TRERT 41_ LVAVK 46_ PDGVQ 51_ RRLVG 56_ DVIQR 61_ FERRG 66_ FTLVG 71_ MKMLQ 76_ APESV 81_ LAEHY 86_ QDLRR 91_ KPFYP 96_ ALIRY 101_ MSSGP 106_ VVAMV 111_ WEGYN 116_ VVRAS 121_ RAMIG 126_ HTDSA 131_ EAAPG 136_ TIRGD 141_ FSVHI 146_ SRNVI 151_ HASDS 156_ VEGAQ 161_ REIQL 166_ WFQSS 171_ ELVSW 176_ ADGGQ 181_HSSIH
1: Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Through the catalyzed exchange of gamma-phosphate between di- and triphosphonucleosides participates in regulation of intracellular nucleotide homeostasis (PubMed:10799505). Binds to anionic phospholipids, predominantly to cardiolipin; the binding inhibits its phosphotransfer activity (PubMed:18635542, PubMed:23150663). Acts as a mitochondria-specific NDK; its association with cardiolipin-containing mitochondrial inner membrane is coupled to respiration suggesting that ADP locally regenerated in the mitochondrion innermembrane space by its activity is directly taken up via ANT ADP/ATP translocase into the matrix space to stimulate respiratory ATP regeneration (PubMed:18635542). Proposed to increase GTP-loading on dynamin-related GTPase OPA1 in mitochondria (PubMed:24970086). In vitro can induce liposome cross-linking suggesting that it can cross-link inner and outer membranes to form contact sites, and promotes intermembrane migration of anionic phosphoplipids. Promotes the redistribution of cardiolipin between the mitochondrial inner membrane and outer membrane which is implicated in pro-apoptotic signaling (PubMed:17028143, PubMed:18635542, PubMed:23150663)