DNA-directed RNA polymerase III subunit RPC7 (RNA polymerase III subunit C7) (DNA-directed RNA polymerase III subunit G) (RNA polymerase III 32 kDa apha subunit) (RPC32-alpha) (RNA polymerase III 32 kDa subunit) (RPC32)
1_MAGNK 6_ GRGRA 11_ AYTFN 16_ IEAVG 21_ FSKGE 26_ KLPDV 31_ VLKPP 36_ PLFPD 41_ TDYKP 46_ VPLKT 51_ GEGEE 56_ YMLAL 61_ KQELR 66_ ETMKR 71_ MPYFI 76_ ETPEE 81_ RQDIE 86_ RYSKR 91_ YMKVY 96_ KEEWI 101_ PDWRR 106_ LPREM 111_ MPRNK 116_ CKKAG 121_ PKPKK 126_ AKDAG 131_ KGTPL 136_ TNTED 141_ VLKKM 146_ EELEK 151_ RGDGE 156_ KSDEE 161_ NEEKE 166_ GSKEK 171_ SKEGD 176_ DDDDD 181_ DAAEQ 186_ EEYDE 191_ EEQEE 196_ ENDYI 201_ NSYFE 206_ DGDDF 211_ GADSD 216_DNMDE
1: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates (PubMed:20413673, PubMed:33558764, PubMed:34675218, PubMed:35637192). Specific peripheric component of RNA polymerase III (Pol III) which synthesizes small non-coding RNAs including 5S rRNA, snRNAs, tRNAs and miRNAs from at least 500 distinct genomic loci (PubMed:20154270, PubMed:20413673, PubMed:35637192). Acts as a long tether that bridges POLR3C/RPC3-POLR3F/RPC6-POLR3G/RPC7 heterotrimer and the mobile stalk of Pol III, coordinating the dynamics of Pol III stalk and clamp modules during the transition from apo to elongation state. Pol III exists as two alternative complexes defined by the mutually exclusive incorporation of subunit POLR3G/RPC7alpha or POLR3GL/RPC7beta. POLR3G/RPC7alpha modulates Pol III transcriptome by specifically enhancing the transcription of snaR-A non-coding RNAs. At resting state, occupies the active site of apo Pol III and keeps Pol III in an autoinhibitory mode, preventing non-specific transcription (PubMed:33558764, PubMed:33558766, PubMed:35637192). Pol III plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as a nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs), induce type I interferon and NF-kappa-B through the RIG-I pathway (PubMed:19609254, PubMed:19631370)