ATPase GET3 (EC 3.6.-.-) (Arsenical pump-driving ATPase) (Arsenite-stimulated ATPase) (Guided entry of tail-anchored proteins factor 3, ATPase) (Transmembrane domain recognition complex 40 kDa ATPase subunit) (hARSA-I) (hASNA-I)
1_MAAGV 6_ AGWGV 11_ EAEEF 16_ EDAPD 21_ VEPLE 26_ PTLSN 31_ IIEQR 36_ SLKWI 41_ FVGGK 46_ GGVGK 51_ TTCSC 56_ SLAVQ 61_ LSKGR 66_ ESVLI 71_ ISTDP 76_ AHNIS 81_ DAFDQ 86_ KFSKV 91_ PTKVK 96_ GYDNL 101_ FAMEI 106_ DPSLG 111_ VAELP 116_ DEFFE 121_ EDNML 126_ SMGKK 131_ MMQEA 136_ MSAFP 141_ GIDEA 146_ MSYAE 151_ VMRLV 156_ KGMNF 161_ SVVVF 166_ DTAPT 171_ GHTLR 176_ LLNFP 181_ TIVER 186_ GLGRL 191_ MQIKN 196_ QISPF 201_ ISQMC 206_ NMLGL 211_ GDMNA 216_ DQLAS 221_ KLEET 226_ LPVIR 231_ SVSEQ 236_ FKDPE 241_ QTTFI 246_ CVCIA 251_ EFLSL 256_ YETER 261_ LIQEL 266_ AKCKI 271_ DTHNI 276_ IVNQL 281_ VFPDP 286_ EKPCK 291_ MCEAR 296_ HKIQA 301_ KYLDQ 306_ MEDLY 311_ EDFHI 316_ VKLPL 321_ LPHEV 326_ RGADK 331_ VNTFS 336_ ALLLE 341_PYKPP
1: ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum (PubMed:17382883). Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors GET1/WRB and CAMLG/GET2, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the GET1-CAMLG receptor, and returning it to the cytosol to initiate a new round of targeting. May be involved in insulin signaling