Maleylacetoacetate isomerase (MAAI) (EC 5.2.1.2) (GSTZ1-1) (Glutathione S-transferase zeta 1) (EC 2.5.1.18)
1_MQAGK 6_ PILYS 11_ YFRSS 16_ CSWRV 21_ RIALA 26_ LKGID 31_ YKTVP 36_ INLIK 41_ DRGQQ 46_ FSKDF 51_ QALNP 56_ MKQVP 61_ TLKID 66_ GITIH 71_ QSLAI 76_ IEYLE 81_ EMRPT 86_ PRLLP 91_ QDPKK 96_ RASVR 101_ MISDL 106_ IAGGI 111_ QPLQN 116_ LSVLK 121_ QVGEE 126_ MQLTW 131_ AQNAI 136_ TCGFN 141_ ALEQI 146_ LQSTA 151_ GIYCV 156_ GDEVT 161_ MADLC 166_ LVPQV 171_ ANAER 176_ FKVDL 181_ TPYPT 186_ ISSIN 191_ KRLLV 196_ LEAFQ 201_ VSHPC 206_ RQPDT 211_PTELR
1: Bifunctional enzyme showing minimal glutathione-conjugating activity with ethacrynic acid and 7-chloro-4-nitrobenz-2-oxa-1,3-diazole and maleylacetoacetate isomerase activity. Also has low glutathione peroxidase activity with T-butyl and cumene hydroperoxides. Is able to catalyze the glutathione dependent oxygenation of dichloroacetic acid to glyoxylic acid