Carbohydrate sulfotransferase 1 (Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 1) (GST-1) (Keratan sulfate Gal-6 sulfotransferase) (KS6ST) (KSGal6ST) (KSST) (EC 2.8.2.21)
1_MQCSW 6_ KAVLL 11_ LALAS 16_ IAIQY 21_ TAIRT 26_ FTAKS 31_ FHTCP 36_ GLAEA 41_ GLAER 46_ LCEES 51_ PTFAY 56_ NLSRK 61_ THILI 66_ LATTR 71_ SGSSF 76_ VGQLF 81_ NQHLD 86_ VFYLF 91_ EPLYH 96_ VQNTL 101_ IPRFT 106_ QGKSP 111_ ADRRV 116_ MLGAS 121_ RDLLR 126_ SLYDC 131_ DLYFL 136_ ENYIK 141_ PPPVN 146_ HTTDR 151_ IFRRG 156_ ASRVL 161_ CSRPV 166_ CDPPG 171_ PADLV 176_ LEEGD 181_ CVRKC 186_ GLLNL 191_ TVAAE 196_ ACRER 201_ SHVAI 206_ KTVRV 211_ PEVND 216_ LRALV 221_ EDPRL 226_ NLKVI 231_ QLVRD 236_ PRGIL 241_ ASRSE 246_ TFRDT 251_ YRLWR 256_ LWYGT 261_ GRKPY 266_ NLDVT 271_ QLTTV 276_ CEDFS 281_ NSVST 286_ GLMRP 291_ PWLKG 296_ KYMLV 301_ RYEDL 306_ ARNPM 311_ KKTEE 316_ IYGFL 321_ GIPLD 326_ SHVAR 331_ WIQNN 336_ TRGDP 341_ TLGKH 346_ KYGTV 351_ RNSAA 356_ TAEKW 361_ RFRLS 366_ YDIVA 371_ FAQNA 376_ CQQVL 381_ AQLGY 386_ KIAAS 391_ EEELK 396_ NPSVS 401_ LVEER 406_DFRPF
1: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of internal galactose (Gal) residues of keratan. Cooperates with B4GALT4 and B3GNT7 glycosyltransferases and CHST6 sulfotransferase to construct and elongate disulfated disaccharide unit [->3(6-sulfoGalbeta)1->4(6-sulfoGlcNAcbeta)1->] within keratan sulfate polymer (PubMed:10642612, PubMed:17690104, PubMed:9405439). Has a preference for sulfating keratan sulfate, but it also transfers sulfate to the unsulfated polymer (PubMed:9405439). Involved in biosynthesis of phosphacan, a major keratan sulfate proteoglycan in the developing brain (By similarity). Involved in biosynthesis of 6-sulfoGalbeta-containing O-linked glycans in high endothelial venules of lymph nodes. May act in a synergistic manner with CHST4 to generate sialyl 6',6-disulfo Lewis X motif, a recognition determinant for immune cell receptors implicated in leukocyte trafficking (PubMed:10330415). Catalyzes sulfation of N-acetyllactosamine (LacNAc) oligosaccharides with highest efficiency for sialylated LacNAc structures (PubMed:10642612)