Protein O-GlcNAcase (OGA) (EC 3.2.1.169) (Beta-N-acetylglucosaminidase) (Beta-N-acetylhexosaminidase) (Beta-hexosaminidase) (Meningioma-expressed antigen 5) (N-acetyl-beta-D-glucosaminidase) (N-acetyl-beta-glucosaminidase) (Nuclear cytoplasmic O-GlcNAcase and acetyltransferase) (NCOAT)
1_MVQKE 6_ SQATL 11_ EERES 16_ ELSSN 21_ PAASA 26_ GASLE 31_ PPAAP 36_ APGED 41_ NPAGA 46_ GGAAV 51_ AGAAG 56_ GARRF 61_ LCGVV 66_ EGFYG 71_ RPWVM 76_ EQRKE 81_ LFRRL 86_ QKWEL 91_ NTYLY 96_ APKDD 101_ YKHRM 106_ FWREM 111_ YSVEE 116_ AEQLM 121_ TLISA 126_ AREYE 131_ IEFIY 136_ AISPG 141_ LDITF 146_ SNPKE 151_ VSTLK 156_ RKLDQ 161_ VSQFG 166_ CRSFA 171_ LLFDD 176_ IDHNM 181_ CAADK 186_ EVFSS 191_ FAHAQ 196_ VSITN 201_ EIYQY 206_ LGEPE 211_ TFLFC 216_ PTEYC 221_ GTFCY 226_ PNVSQ 231_ SPYLR 236_ TVGEK 241_ LLPGI 246_ EVLWT 251_ GPKVV 256_ SKEIP 261_ VESIE 266_ EVSKI 271_ IKRAP 276_ VIWDN 281_ IHAND 286_ YDQKR 291_ LFLGP 296_ YKGRS 301_ TELIP 306_ RLKGV 311_ LTNPN 316_ CEFEA 321_ NYVAI 326_ HTLAT 331_ WYKSN 336_ MNGVR 341_ KDVVM 346_ TDSED 351_ STVSI 356_ QIKLE 361_ NEGSD 366_ EDIET 371_ DVLYS 376_ PQMAL 381_ KLALT 386_ EWLQE 391_ FGVPH 396_ QYSSR 401_ QVAHS 406_ GAKAS 411_ VVDGT 416_ PLVAA 421_ PSLNA 426_ TTVVT 431_ TVYQE 436_ PIMSQ 441_ GAALS 446_ GEPTT 451_ LTKEE 456_ EKKQP 461_ DEEPM 466_ DMVVE 471_ KQEET 476_ DHKND 481_ NQILS 486_ EIVEA 491_ KMAEE 496_ LKPMD 501_ TDKES 506_ IAESK 511_ SPEMS 516_ MQEDC 521_ ISDIA 526_ PMQTD 531_ EQTNK 536_ EQFVP 541_ GPNEK 546_ PLYTA 551_ EPVTL 556_ EDLQL 561_ LADLF 566_ YLPYE 571_ HGPKG 576_ AQMLR 581_ EFQWL 586_ RANSS 591_ VVSVN 596_ CKGKD 601_ SEKIE 606_ EWRSR 611_ AAKFE 616_ EMCGL 621_ VMGMF 626_ TRLSN 631_ CANRT 636_ ILYDM 641_ YSYVW 646_ DIKSI 651_ MSMVK 656_ SFVQW 661_ LGCRS 666_ HSSAQ 671_ FLIGD 676_ QEPWA 681_ FRGGL 686_ AGEFQ 691_ RLLPI 696_ DGAND 701_ LFFQP 706_ PPLTP 711_ TSKVY 716_ TIRPY 721_ FPKDE 726_ ASVYK 731_ ICREM 736_ YDDGV 741_ GLPFQ 746_ SQPDL 751_ IGDKL 756_ VGGLL 761_ SLSLD 766_ YCFVL 771_ EDEDG 776_ ICGYA 781_ LGTVD 786_ VTPFI 791_ KKCKI 796_ SWIPF 801_ MQEKY 806_ TKPNG 811_ DKELS 816_ EAEKI 821_ MLSFH 826_ EEQEV 831_ LPETF 836_ LANFP 841_ SLIKM 846_ DIHKK 851_ VTDPS 856_ VAKSM 861_ MACLL 866_ SSLKA 871_ NGSRG 876_ AFCEV 881_ RPDDK 886_ RILEF 891_ YSKLG 896_ CFEIA 901_ KMEGF 906_ PKDVV 911_ILGRS
1: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins (PubMed:11148210, PubMed:11788610, PubMed:20673219, PubMed:22365600, PubMed:24088714, PubMed:28939839, PubMed:37962578). Deglycosylates a large and diverse number of proteins, such as CRYAB, ELK1, GSDMD, LMNB1 and TAB1 (PubMed:28939839, PubMed:37962578). Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro) (PubMed:20673219). Does not bind acetyl-CoA and does not have histone acetyltransferase activity (PubMed:24088714)
2: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro), but has about six times lower specific activity than isoform 1