Ribosome quality control complex subunit NEMF (Antigen NY-CO-1) (Nuclear export mediator factor) (Serologically defined colon cancer antigen 1)
1_MKSRF 6_ STIDL 11_ RAVLA 16_ ELNAS 21_ LLGMR 26_ VNNVY 31_ DVDNK 36_ TYLIR 41_ LQKPD 46_ FKATL 51_ LLESG 56_ IRIHT 61_ TEFEW 66_ PKNMM 71_ PSSFA 76_ MKCRK 81_ HLKSR 86_ RLVSA 91_ KQLGV 96_ DRIVD 101_ FQFGS 106_ DEAAY 111_ HLIIE 116_ LYDRG 121_ NIVLT 126_ DYEYV 131_ ILNIL 136_ RFRTD 141_ EADDV 146_ KFAVR 151_ ERYPL 156_ DHARA 161_ AEPLL 166_ TLERL 171_ TEIVA 176_ SAPKG 181_ ELLKR 186_ VLNPL 191_ LPYGP 196_ ALIEH 201_ CLLEN 206_ GFSGN 211_ VKVDE 216_ KLETK 221_ DIEKV 226_ LVSLQ 231_ KAEDY 236_ MKTTS 241_ NFSGK 246_ GYIIQ 251_ KREIK 256_ PSLEA 261_ DKPVE 266_ DILTY 271_ EEFHP 276_ FLFSQ 281_ HSQCP 286_ YIEFE 291_ SFDKA 296_ VDEFY 301_ SKIEG 306_ QKIDL 311_ KALQQ 316_ EKQAL 321_ KKLDN 326_ VRKDH 331_ ENRLE 336_ ALQQA 341_ QEIDK 346_ LKGEL 351_ IEMNL 356_ QIVDR 361_ AIQVV 366_ RSALA 371_ NQIDW 376_ TEIGL 381_ IVKEA 386_ QAQGD 391_ PVASA 396_ IKELK 401_ LQTNH 406_ VTMLL 411_ RNPYL 416_ LSEEE 421_ DDDVD 426_ GDVNV 431_ EKNET 436_ EPPKG 441_ KKKKQ 446_ KNKQL 451_ QKPQK 456_ NKPLL 461_ VDVDL 466_ SLSAY 471_ ANAKK 476_ YYDHK 481_ RYAAK 486_ KTQKT 491_ VEAAE 496_ KAFKS 501_ AEKKT 506_ KQTLK 511_ EVQTV 516_ TSIQK 521_ ARKVY 526_ WFEKF 531_ LWFIS 536_ SENYL 541_ IIGGR 546_ DQQQN 551_ EIIVK 556_ RYLTP 561_ GDIYV 566_ HADLH 571_ GATSC 576_ VIKNP 581_ TGEPI 586_ PPRTL 591_ TEAGT 596_ MALCY 601_ SAAWD 606_ ARVIT 611_ SAWWV 616_ YHHQV 621_ SKTAP 626_ TGEYL 631_ TTGSF 636_ MIRGK 641_ KNFLP 646_ PSYLM 651_ MGFSF 656_ LFKVD 661_ ESCVW 666_ RHQGE 671_ RKVRV 676_ QDEDM 681_ ETLAS 686_ CTSEL 691_ ISEEM 696_ EQLDG 701_ GDTSS 706_ DEDKE 711_ EHETP 716_ VEVEL 721_ MTQVD 726_ QEDIT 731_ LQSGR 736_ DELNE 741_ ELIQE 746_ ESSED 751_ EGEYE 756_ EVRKD 761_ QDSVG 766_ EMKDE 771_ GEETL 776_ NYPDT 781_ TIDLS 786_ HLQPQ 791_ RSIQK 796_ LASKE 801_ ESSNS 806_ SDSKS 811_ QSRRH 816_ LSAKE 821_ RREMK 826_ KKKLP 831_ SDSGD 836_ LEALE 841_ GKDKE 846_ KESTV 851_ HIETH 856_ QNTSK 861_ NVAAV 866_ QPMKR 871_ GQKSK 876_ MKKMK 881_ EKYKD 886_ QDEED 891_ RELIM 896_ KLLGS 901_ AGSNK 906_ EEKGK 911_ KGKKG 916_ KTKDE 921_ PVKKQ 926_ PQKPR 931_ GGQRV 936_ SDNIK 941_ KETPF 946_ LEVIT 951_ HELQD 956_ FAVDD 961_ PHDDK 966_ EEQDL 971_ DQQGN 976_ EENLF 981_ DSLTG 986_ QPHPE 991_ DVLLF 996_ AIPIC 1001_ APYTT 1006_ MTNYK 1011_ YKVKL 1016_ TPGVQ 1021_ KKGKA 1026_ AKTAL 1031_ NSFMH 1036_ SKEAT 1041_ AREKD 1046_ LFRSV 1051_ KDTDL 1056_ SRNIP 1061_ GKVKV 1066_ SAPNL 1071_LNVKR
1: Key component of the ribosome quality control complex (RQC), a ribosome-associated complex that mediates the extraction of incompletely synthesized nascent chains from stalled ribosomes as well as their ubiquitin-mediated proteasomal degradation (PubMed:25578875, PubMed:32726578, PubMed:33406423, PubMed:33909987). Thereby, frees 60S subunit ribosomes from the stalled translation complex and prevents the accumulation of nascent polypeptide chains that are potentially toxic for the cell (PubMed:25578875, PubMed:33406423, PubMed:33909987). Within the RQC complex, NEMF specifically binds stalled 60S ribosomal subunits by recognizing an exposed, nascent chain-conjugated tRNA moiety and promotes the recruitment of LTN1 to stalled 60S subunits (PubMed:25578875). Following binding to stalled 60S ribosomal subunits, NEMF mediates CAT tailing by recruiting alanine-charged tRNA to the A-site and directing the elongation of stalled nascent chains independently of mRNA or 40S subunits, leading to non-templated C-terminal alanine extensions (CAT tails) (PubMed:33406423, PubMed:33909987). Mainly recruits alanine-charged tRNAs, but can also other amino acid-charged tRNAs (PubMed:33406423, PubMed:33909987). CAT tailing is required to promote ubiquitination of stalled nascent chains by different E3 ubiquitin-protein ligases (PubMed:33909987). In the canonical RQC pathway (RQC-L), CAT tailing facilitates LTN1-dependent ubiquitination by exposing lysine residues that would otherwise remain buried in the ribosomal exit tunnel (By similarity). In the alternative RQC pathway (RQC-C) CAT tailing creates an C-degron mainly composed of alanine that is recognized by the CRL2(KLHDC10) and RCHY1/PIRH2 E3 ligases, leading to ubiquitination and degradation of stalled nascent chains (PubMed:33909987). NEMF may also indirectly play a role in nuclear export (PubMed:16103875)