Putative tyrosine-protein phosphatase auxilin (EC 3.1.3.48) (DnaJ homolog subfamily C member 6)
1_MKDSE 6_ NKGAS 11_ SPDME 16_ PSYGG 21_ GLFDM 26_ VKGGA 31_ GRLFS 36_ NLKDN 41_ LKDTL 46_ KDTSS 51_ RVIQS 56_ VTSYT 61_ KGDLD 66_ FTYVT 71_ SRIIV 76_ MSFPL 81_ DNVDI 86_ GFRNQ 91_ VDDIR 96_ SFLDS 101_ RHLDH 106_ YTVYN 111_ LSPKS 116_ YRTAK 121_ FHSRV 126_ SECSW 131_ PIRQA 136_ PSLHN 141_ LFAVC 146_ RNMYN 151_ WLLQN 156_ PKNVC 161_ VVHCL 166_ DGRAA 171_ SSILV 176_ GAMFI 181_ FCNLY 186_ STPGP 191_ AIRLL 196_ YAKRP 201_ GIGLS 206_ PSHRR 211_ YLGYM 216_ CDLLA 221_ DKPYR 226_ PHFKP 231_ LTIKS 236_ ITVSP 241_ IPFFN 246_ KQRNG 251_ CRPYC 256_ DVLIG 261_ ETKIY 266_ STCTD 271_ FERMK 276_ EYRVQ 281_ DGKIF 286_ IPLNI 291_ TVQGD 296_ VVVSM 301_ YHLRS 306_ TIGSR 311_ LQAKV 316_ TNTQI 321_ FQLQF 326_ HTGFI 331_ PLDTT 336_ VLKFT 341_ KPELD 346_ ACDVP 351_ EKYPQ 356_ LFQVT 361_ LDVEL 366_ QPHDK 371_ VIDLT 376_ PPWEH 381_ YCTKD 386_ VNPSI 391_ LFSSH 396_ QEHQD 401_ TLALG 406_ GQAPI 411_ DIPPD 416_ NPRHY 421_ GQSGF 426_ FASLC 431_ WQDQK 436_ SEKSF 441_ CEEDH 446_ AALVN 451_ QESEQ 456_ SDDEL 461_ LTLSS 466_ PHGNA 471_ NGDKP 476_ HGVKK 481_ PSKKQ 486_ QEPAA 491_ PPPPE 496_ DVDLL 501_ GLEGS 506_ AMSNS 511_ FSPPA 516_ APPTN 521_ SELLS 526_ DLFGG 531_ GGAAG 536_ PTQAG 541_ QSGVE 546_ DVFHP 551_ SGPAS 556_ TQSTP 561_ RRSAT 566_ STSAS 571_ PTLRV 576_ GEGAT 581_ FDPFG 586_ APSKP 591_ SGQDL 596_ LGSFL 601_ NTSSA 606_ SSDPF 611_ LQPTR 616_ SPSPT 621_ VHASS 626_ TPAVN 631_ IQPDV 636_ SGGWD 641_ WHAKP 646_ GGFGM 651_ GSKSA 656_ ATSPT 661_ GSSHG 666_ TPTHQ 671_ SKPQT 676_ LDPFA 681_ DLGTL 686_ GSSSF 691_ ASKPT 696_ TPTGL 701_ GGGFP 706_ PLSSP 711_ QKASP 716_ QPMGG 721_ GWQQG 726_ GAYNW 731_ QQPQP 736_ KPQPS 741_ MPHSS 746_ PQNRP 751_ NYNVS 756_ FSAMP 761_ GGQNE 766_ RGKGS 771_ SNLEG 776_ KQKAA 781_ DFEDL 786_ LSGQG 791_ FNAHK 796_ DKKGP 801_ RTIAE 806_ MRKEE 811_ MAKEM 816_ DPEKL 821_ KILEW 826_ IEGKE 831_ RNIRA 836_ LLSTM 841_ HTVLW 846_ AGETK 851_ WKPVG 856_ MADLV 861_ TPEQV 866_ KKVYR 871_ KAVLV 876_ VHPDK 881_ ATGQP 886_ YEQYA 891_ KMIFM 896_ ELNDA 901_ WSEFE 906_NQGQK
1: May act as a protein phosphatase and/or a lipid phosphatase. Co-chaperone that recruits HSPA8/HSC70 to clathrin-coated vesicles (CCVs) and promotes the ATP-dependent dissociation of clathrin from CCVs and participates in clathrin-mediated endocytosis of synaptic vesicles and their recycling and also in intracellular trafficking (PubMed:18489706). Firstly, binds tightly to the clathrin cages, at a ratio of one DNAJC6 per clathrin triskelion. The HSPA8:ATP complex then binds to the clathrin-auxilin cage, initially at a ratio of one HSPA8 per triskelion leading to ATP hydrolysis stimulation and causing a conformational change in the HSPA8. This cycle is repeated three times to drive to a complex containing the clathrin-auxilin cage associated to three HSPA8:ADP complex. The ATP hydrolysis of the third HSPA8:ATP complex leads to a concerted dismantling of the cage into component triskelia. Then, dissociates from the released triskelia and be recycled to initiate another cycle of HSPA8's recruitment. Also acts during the early steps of clathrin-coated vesicle (CCV) formation through its interaction with the GTP bound form of DNM1 (By similarity)