GATOR complex protein DEPDC5 (DEP domain-containing protein 5)
1_MRTTK 6_ VYKLV 11_ IHKKG 16_ FGGSD 21_ DELVV 26_ NPKVF 31_ PHIKL 36_ GDIVE 41_ IAHPN 46_ DEYSP 51_ LLLQV 56_ KSLKE 61_ DLQKE 66_ TISVD 71_ QTVTQ 76_ VFRLR 81_ PYQDV 86_ YVNVV 91_ DPKDV 96_ TLDLV 101_ ELTFK 106_ DQYIG 111_ RGDMW 116_ RLKKS 121_ LVSTC 126_ AYITQ 131_ KVEFA 136_ GIRAQ 141_ AGELW 146_ VKNEK 151_ VMCGY 156_ ISEDT 161_ RVVFR 166_ STSAM 171_ VYIFI 176_ QMSCE 181_ MWDFD 186_ IYGDL 191_ YFEKA 196_ VNGFL 201_ ADLFT 206_ KWKEK 211_ NCSHE 216_ VTVVL 221_ FSRTF 226_ YDAKS 231_ VDEFP 236_ EINRA 241_ SIRQD 246_ HKGRF 251_ YEDFY 256_ KVVVQ 261_ NERRE 266_ EWTSL 271_ LVTIK 276_ KLFIQ 281_ YPVLV 286_ RLEQA 291_ EGFPQ 296_ GDNST 301_ SAQGN 306_ YLEAI 311_ NLSFN 316_ VFDKH 321_ YINRN 326_ FDRTG 331_ QMSVV 336_ ITPGV 341_ GVFEV 346_ DRLLM 351_ ILTKQ 356_ RMIDN 361_ GIGVD 366_ LVCMG 371_ EQPLH 376_ AVPLF 381_ KLHNR 386_ SAPRD 391_ SRLGD 396_ DYNIP 401_ HWINH 406_ SFYTS 411_ KSQLF 416_ CNSFT 421_ PRIKL 426_ AGKKP 431_ ASEKA 436_ KNGRD 441_ TSLGS 446_ PKESE 451_ NALPI 456_ QVDYD 461_ AYDAQ 466_ VFRLP 471_ GPSRA 476_ QCLTT 481_ CRSVR 486_ ERESH 491_ SRKSA 496_ SSCDV 501_ SSSPS 506_ LPSRT 511_ LPTEE 516_ VRSQA 521_ SDDSS 526_ LGKSA 531_ NILMI 536_ PHPHL 541_ HQYEV 546_ SSSLG 551_ YTSTR 556_ DVLEN 561_ MMEPP 566_ QRDSS 571_ APGRF 576_ HVGSA 581_ ESMLH 586_ VRPGG 591_ YTPQR 596_ ALINP 601_ FAPSR 606_ MPMKL 611_ TSNRR 616_ RWMHT 621_ FPVGP 626_ SGEAI 631_ QIHHQ 636_ TRQNM 641_ AELQG 646_ SGQRD 651_ PTHSS 656_ AELLE 661_ LAYHE 666_ AAGRH 671_ SNSRQ 676_ PGDGM 681_ SFLNF 686_ SGTEE 691_ LSVGL 696_ LSNSG 701_ AGMNP 706_ RTQNK 711_ DSLED 716_ SVSTS 721_ PDPIL 726_ TLSAP 731_ PVVPG 736_ FCCTV 741_ GVDWK 746_ SLTTP 751_ ACLPL 756_ TTDYF 761_ PDRQG 766_ LQNDY 771_ TEGCY 776_ DLLPE 781_ ADIDR 786_ RDEDG 791_ VQMTA 796_ QQVFE 801_ EFICQ 806_ RLMQG 811_ YQIIV 816_ QPKTQ 821_ KPNPA 826_ VPPPL 831_ SSSPL 836_ YSRGL 841_ VSRNR 846_ PEEED 851_ QYWLS 856_ MGRTF 861_ HKVTL 866_ KDKMI 871_ TVTRY 876_ LPKYP 881_ YESAQ 886_ IHYTY 891_ SLCPS 896_ HSDSE 901_ FVSCW 906_ VEFSH 911_ ERLEE 916_ YKWNY 921_ LDQYI 926_ CSAGS 931_ EDFSL 936_ IESLK 941_ FWRTR 946_ FLLLP 951_ ACVTA 956_ TKRIT 961_ EGEAH 966_ CDIYG 971_ DRPRA 976_ DEDEW 981_ QLLDG 986_ FVRFV 991_ EGLNR 996_ IRRRH 1001_ RSDRM 1006_ MRKGT 1011_ AMKGL 1016_ QMTGP 1021_ ISTHS 1026_ LESTA 1031_ PPVGK 1036_ KGTSA 1041_ LSALL 1046_ EMEAS 1051_ QKCLG 1056_ EQQAA 1061_ VHGGK 1066_ SSAQS 1071_ AESSS 1076_ VAMTP 1081_ TYMDS 1086_ PRKDG 1091_ AFFME 1096_ FVRSP 1101_ RTASS 1106_ AFYPQ 1111_ VSVDQ 1116_ TATPM 1121_ LDGTS 1126_ LGICT 1131_ GQSMD 1136_ RGNSQ 1141_ TFGNS 1146_ QNIGE 1151_ QGYSS 1156_ TNSSD 1161_ SSSQQ 1166_ LVASS 1171_ LTSSS 1176_ TLTEI 1181_ LEAMK 1186_ HPSTG 1191_ VQLLS 1196_ EQKGL 1201_ SPYCF 1206_ ISAEV 1211_ VHWLV 1216_ NHVEG 1221_ IQTQA 1226_ MAIDI 1231_ MQKML 1236_ EEQLI 1241_ THASG 1246_ EAWRT 1251_ FIYGF 1256_ YFYKI 1261_ VTDKE 1266_ PDRVA 1271_ MQQPA 1276_ TTWHT 1281_ AGVDD 1286_ FASFQ 1291_ RKWFE 1296_ VAFVA 1301_ EELVH 1306_ SEIPA 1311_ FLLPW 1316_ LPSRP 1321_ ASYAS 1326_ RHSSF 1331_ SRSFG 1336_ GRSQA 1341_ AALLA 1346_ ATVPE 1351_ QRTVT 1356_ LDVDV 1361_ NNRTD 1366_ RLEWC 1371_ SCYYH 1376_ GNFSL 1381_ NAAFE 1386_ IKLHW 1391_ MAVTA 1396_ AVLFE 1401_ MVQGW 1406_ HRKAT 1411_ SCGFL 1416_ LVPVL 1421_ EGPFA 1426_ LPSYL 1431_ YGDPL 1436_ RAQLF 1441_ IPLNI 1446_ SCLLK 1451_ EGSEH 1456_ LFDSF 1461_ EPETY 1466_ WDRMH 1471_ LFQEA 1476_ IAHRF 1481_ GFVQD 1486_ KYSAS 1491_ AFNFP 1496_ AENKP 1501_ QYIHV 1506_ TGTVF 1511_ LQLPY 1516_ SKRKF 1521_ SGQQR 1526_ RRRNS 1531_ TSSTN 1536_ QNMFC 1541_ EERVG 1546_ YNWAY 1551_ NTMLT 1556_ KTWRS 1561_ SATGD 1566_ EKFAD 1571_ RLLKD 1576_ FTDFC 1581_ INRDN 1586_ RLVTF 1591_ WTSCL 1596_EKMHA
1: As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the mTORC1 pathway (PubMed:23723238, PubMed:25457612, PubMed:29590090, PubMed:29769719, PubMed:31548394, PubMed:35338845). In response to amino acid depletion, the GATOR1 complex has GTPase activating protein (GAP) activity and strongly increases GTP hydrolysis by RagA/RRAGA (or RagB/RRAGB) within heterodimeric Rag complexes, thereby turning them into their inactive GDP-bound form, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling (PubMed:23723238, PubMed:25457612, PubMed:29590090, PubMed:29769719, PubMed:35338845). In the presence of abundant amino acids, the GATOR1 complex is negatively regulated by GATOR2, the other GATOR subcomplex, in this amino acid-sensing branch of the TORC1 pathway (PubMed:23723238, PubMed:25457612, PubMed:29769719). Within the GATOR1 complex, DEPDC5 mediates direct interaction with the nucleotide-binding pocket of small GTPases Rag (RagA/RRAGA, RagB/RRAGB, RagC/RRAGC and/or RagD/RRAGD) and coordinates their nucleotide loading states by promoting RagA/RRAGA or RagB/RRAGB into their GDP-binding state and RagC/RRAGC or RagD/RRAGD into their GTP-binding state (PubMed:29590090, PubMed:35338845). However, it does not execute the GAP activity, which is mediated by NPRL2 (PubMed:29590090)