Acyl-protein thioesterase 1 (APT-1) (hAPT1) (EC 3.1.2.-) (Lysophospholipase 1) (Lysophospholipase I) (LPL-I) (LysoPLA I) (Palmitoyl-protein hydrolase) (EC 3.1.2.22)
1_MCGNN 6_ MSTPL 11_ PAIVP 16_ AARKA 21_ TAAVI 26_ FLHGL 31_ GDTGH 36_ GWAEA 41_ FAGIR 46_ SSHIK 51_ YICPH 56_ APVRP 61_ VTLNM 66_ NVAMP 71_ SWFDI 76_ IGLSP 81_ DSQED 86_ ESGIK 91_ QAAEN 96_ IKALI 101_ DQEVK 106_ NGIPS 111_ NRIIL 116_ GGFSQ 121_ GGALS 126_ LYTAL 131_ TTQQK 136_ LAGVT 141_ ALSCW 146_ LPLRA 151_ SFPQG 156_ PIGGA 161_ NRDIS 166_ ILQCH 171_ GDCDP 176_ LVPLM 181_ FGSLT 186_ VEKLK 191_ TLVNP 196_ ANVTF 201_ KTYEG 206_ MMHSS 211_ CQQEM 216_ MDVKQ 221_FIDKL
1: Acts as an acyl-protein thioesterase (PubMed:19439193, PubMed:20418879). Hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS (PubMed:20418879). Acts as a palmitoyl thioesterase that catalyzes depalmitoylation of proteins, such as ADRB2, KCNMA1 and SQSTM1 (PubMed:22399288, PubMed:27481942, PubMed:37802024). Acts as a negative regulator of autophagy by mediating palmitoylation of SQSTM1, decreasing affinity between SQSTM1 and ATG8 proteins and recruitment of ubiquitinated cargo proteins to autophagosomes (PubMed:37802024). Acts as a lysophospholipase and hydrolyzes lysophosphatidylcholine (lyso-PC) (PubMed:19439193). Also hydrolyzes lysophosphatidylethanolamine (lyso-PE), lysophosphatidylinositol (lyso-PI) and lysophosphatidylserine (lyso-PS) (By similarity). Has much higher thioesterase activity than lysophospholipase activity (PubMed:19439193). Contributes to the production of lysophosphatidic acid (LPA) during blood coagulation by recognizing and cleaving plasma phospholipids to generate lysophospholipids which in turn act as substrates for ENPP2 to produce LPA (PubMed:21393252)