Mitochondrial tRNA-specific 2-thiouridylase 1 (EC 2.8.1.14) (MTO2 homolog)
1_MQALR 6_ HVVCA 11_ LSGGV 16_ DSAVA 21_ ALLLR 26_ RRGYQ 31_ VTGVF 36_ MKNWD 41_ SLDEH 46_ GVCTA 51_ DKDCE 56_ DAYRV 61_ CQILD 66_ IPFHQ 71_ VSYVK 76_ EYWND 81_ VFSDF 86_ LNEYE 91_ KGRTP 96_ NPDIV 101_ CNKHI 106_ KFSCF 111_ FHYAV 116_ DNLGA 121_ DAIAT 126_ GHYAR 131_ TSLED 136_ EEVFE 141_ QKHVK 146_ KPEGL 151_ FRNRF 156_ EVRNA 161_ VKLLQ 166_ AADSF 171_ KDQTF 176_ FLSQV 181_ SQDAL 186_ RRTIF 191_ PLGGL 196_ TKEFV 201_ KKIAA 206_ ENRLH 211_ HVLQK 216_ KESMG 221_ MCFIG 226_ KRNFE 231_ HFLLQ 236_ YLQPR 241_ PGHFI 246_ SIEDN 251_ KVLGT 256_ HKGWF 261_ LYTLG 266_ QRANI 271_ GGLRE 276_ PWYVV 281_ EKDSV 286_ KGDVF 291_ VAPRT 296_ DHPAL 301_ YRDLL 306_ RTSRV 311_ HWIAE 316_ EPPAA 321_ LVRDK 326_ MMECH 331_ FRFRH 336_ QMALV 341_ PCVLT 346_ LNQDG 351_ TVWVT 356_ AVQAV 361_ RALAT 366_ GQFAV 371_ FYKGD 376_ ECLGS 381_ GKILR 386_ LGPSA 391_ YTLQK 396_ GQRRA 401_ GMATE 406_ SPSDS 411_ PEDGP 416_GLSPL
1: Catalyzes the 2-thiolation of uridine at the wobble position (U34) of mitochondrial tRNA(Lys), tRNA(Glu) and tRNA(Gln). Required for the formation of 5-taurinomethyl-2-thiouridine (tm5s2U) of mitochondrial tRNA(Lys), tRNA(Glu), and tRNA(Gln) at the wobble position. ATP is required to activate the C2 atom of the wobble base