Decapping and exoribonuclease protein (DXO) (EC 3.6.1.-) (5'-3' exoribonuclease DXO) (EC 3.1.13.-) (Dom-3 homolog Z) (NAD-capped RNA hydrolase DXO) (DeNADding enzyme DXO) (EC 3.6.1.-)
1_MDPRG 6_ TKRGA 11_ EKTEV 16_ AEPRN 21_ KLPRP 26_ APSLP 31_ TDPAL 36_ YSGPF 41_ PFYRR 46_ PSELG 51_ CFSLD 56_ AQRQY 61_ HGDAR 66_ ALRYY 71_ SPPPT 76_ NGPGP 81_ NFDLR 86_ DGYPD 91_ RYQPR 96_ DEEVQ 101_ ERLDH 106_ LLCWL 111_ LEHRG 116_ RLEGG 121_ PGWLA 126_ EAIVT 131_ WRGHL 136_ TKLLT 141_ TPYER 146_ QEGWQ 151_ LAASR 156_ FQGTL 161_ YLSEV 166_ ETPNA 171_ RAQRL 176_ ARPPL 181_ LRELM 186_ YMGYK 191_ FEQYM 196_ CADKP 201_ GSSPD 206_ PSGEV 211_ NTNVA 216_ FCSVL 221_ RSRLG 226_ SHPLL 231_ FSGEV 236_ DCTDP 241_ QAPST 246_ QPPTC 251_ YVELK 256_ TSKEM 261_ HSPGQ 266_ WRSFY 271_ RHKLL 276_ KWWAQ 281_ SFLPG 286_ VPNVV 291_ AGFRN 296_ PDGFV 301_ SSLKT 306_ FPTMK 311_ MFEYV 316_ RNDRD 321_ GWNPS 326_ VCMNF 331_ CAAFL 336_ SFAQS 341_ TVVQD 346_ DPRLV 351_ HLFSW 356_ EPGGP 361_ VTVSV 366_ HQDAP 371_ YAFLP 376_ IWYVE 381_ AMTQD 386_ LPSPP 391_KTPSP
1: Decapping enzyme for NAD-capped RNAs: specifically hydrolyzes the nicotinamide adenine dinucleotide (NAD) cap from a subset of RNAs by removing the entire NAD moiety from the 5'-end of an NAD-capped RNA (PubMed:28283058). The NAD-cap is present at the 5'-end of some RNAs and snoRNAs (PubMed:28283058). In contrast to the canonical 5'-end N7 methylguanosine (m7G) cap, the NAD cap promotes mRNA decay (PubMed:28283058). Preferentially acts on NAD-capped transcripts in response to environmental stress (PubMed:31101919). Also acts as a non-canonical decapping enzyme that removes the entire cap structure of m7G capped or incompletely capped RNAs and mediates their subsequent degradation (By similarity). Specifically degrades pre-mRNAs with a defective 5'-end m7G cap and is part of a pre-mRNA capping quality control (By similarity). Has decapping activity toward incomplete 5'-end m7G cap mRNAs such as unmethylated 5'-end-capped RNA (cap0), while it has no activity toward 2'-O-ribose methylated m7G cap (cap1) (PubMed:29601584). In contrast to canonical decapping enzymes DCP2 and NUDT16, which cleave the cap within the triphosphate linkage, the decapping activity releases the entire cap structure GpppN and a 5'-end monophosphate RNA (By similarity). Also has 5'-3' exoribonuclease activities: The 5'-end monophosphate RNA is then degraded by the 5'-3' exoribonuclease activity, enabling this enzyme to decap and degrade incompletely capped mRNAs (PubMed:29601584). Also possesses RNA 5'-pyrophosphohydrolase activity by hydrolyzing the 5'-end triphosphate to release pyrophosphates (By similarity). Exhibits decapping activity towards FAD-capped RNAs (PubMed:32374864). Exhibits decapping activity towards dpCoA-capped RNAs in vitro (By similarity)