ZW10 interactor (ZW10-interacting protein 1) (Zwint-1)
1_MEAAE 6_ TEAEA 11_ AALEV 16_ LAEVA 21_ GILEP 26_ VGLQE 31_ EAELP 36_ AKILV 41_ EFVVD 46_ SQKKD 51_ KLLCS 56_ QLQVA 61_ DFLQN 66_ ILAQE 71_ DTAKG 76_ LDPLA 81_ SEDTS 86_ RQKAI 91_ AAKEQ 96_ WKELK 101_ ATYRE 106_ HVEAI 111_ KIGLT 116_ KALTQ 121_ MEEAQ 126_ RKRTQ 131_ LREAF 136_ EQLQA 141_ KKQMA 146_ MEKRR 151_ AVQNQ 156_ WQLQQ 161_ EKHLQ 166_ HLAEV 171_ SAEVR 176_ ERKTG 181_ TQQEL 186_ DRVFQ 191_ KLGNL 196_ KQQAE 201_ QERDK 206_ LQRYQ 211_ TFLQL 216_ LYTLQ 221_ GKLLF 226_ PEAEA 231_ EAENL 236_ PDDKP 241_ QQPTR 246_ PQEQS 251_ TGDTM 256_ GRDPG 261_ VSFKA 266_ VGLQP 271_AGDVN
1: Acts as a component of the outer kinetochore KNL1 complex that serves as a docking point for spindle assembly checkpoint components and mediates microtubule-kinetochore interactions (PubMed:15094189, PubMed:15485811, PubMed:15824131, PubMed:16732327, PubMed:24530301, PubMed:27881301, PubMed:38459127, PubMed:38459128). Kinetochores, consisting of a centromere-associated inner segment and a microtubule-contacting outer segment, play a crucial role in chromosome segregation by mediating the physical connection between centromeric DNA and spindle microtubules (PubMed:15094189, PubMed:15485811, PubMed:16732327). The outer kinetochore is made up of the ten-subunit KMN network, comprising the MIS12, NDC80 and KNL1 complexes, and auxiliary microtubule-associated components; together they connect the outer kinetochore with the inner kinetochore, bind microtubules, and mediate interactions with mitotic checkpoint proteins that delay anaphase until chromosomes are bioriented on the spindle (PubMed:15094189, PubMed:15485811, PubMed:15824131, PubMed:16732327, PubMed:24530301, PubMed:38459127, PubMed:38459128). Targets the RZZ complex to the kinetochore at prometaphase (PubMed:15485811). Recruits MAD2L1 to the kinetochore, but is not required for BUB1B localization (By similarity). In addition to orienting mitotic chromosomes, it is also essential for alignment of homologous chromosomes during meiotic metaphase I (By similarity). In meiosis I, required to activate the spindle assembly checkpoint at unattached kinetochores to correct erroneous kinetochore-microtubule attachments (PubMed:15485811)