E3 ubiquitin-protein ligase ARIH2 (ARI-2) (Protein ariadne-2 homolog) (EC 2.3.2.31) (RING-type E3 ubiquitin transferase ARIH2) (Triad1 protein)
1_MSVDM 6_ NSQGS 11_ DSNEE 16_ DYDPN 21_ CEEEE 26_ EEEED 31_ DPGDI 36_ EDYYV 41_ GVASD 46_ VEQQG 51_ ADAFD 56_ PEEYQ 61_ FTCLT 66_ YKESE 71_ GALNE 76_ HMTSL 81_ ASVLK 86_ VSHSV 91_ AKLIL 96_ VNFHW 101_ QVSEI 106_ LDRYK 111_ SNSAQ 116_ LLVEA 121_ RVQPN 126_ PSKHV 131_ PTSHP 136_ PHHCA 141_ VCMQF 146_ VRKEN 151_ LLSLA 156_ CQHQF 161_ CRSCW 166_ EQHCS 171_ VLVKD 176_ GVGVG 181_ VSCMA 186_ QDCPL 191_ RTPED 196_ FVFPL 201_ LPNEE 206_ LREKY 211_ RRYLF 216_ RDYVE 221_ SHYQL 226_ QLCPG 231_ ADCPM 236_ VIRVQ 241_ EPRAR 246_ RVQCN 251_ RCNEV 256_ FCFKC 261_ RQMYH 266_ APTDC 271_ ATIRK 276_ WLTKC 281_ ADDSE 286_ TANYI 291_ SAHTK 296_ DCPKC 301_ NICIE 306_ KNGGC 311_ NHMQC 316_ SKCKH 321_ DFCWM 326_ CLGDW 331_ KTHGS 336_ EYYEC 341_ SRYKE 346_ NPDIV 351_ NQSQQ 356_ AQARE 361_ ALKKY 366_ LFYFE 371_ RWENH 376_ NKSLQ 381_ LEAQT 386_ YQRIH 391_ EKIQE 396_ RVMNN 401_ LGTWI 406_ DWQYL 411_ QNAAK 416_ LLAKC 421_ RYTLQ 426_ YTYPY 431_ AYYME 436_ SGPRK 441_ KLFEY 446_ QQAQL 451_ EAEIE 456_ NLSWK 461_ VERAD 466_ SYDRG 471_ DLENQ 476_ MHIAE 481_ QRRRT 486_LLKDF
1: E3 ubiquitin-protein ligase, which catalyzes ubiquitination of target proteins together with ubiquitin-conjugating enzyme E2 UBE2L3 (PubMed:16118314, PubMed:17646546, PubMed:19340006, PubMed:24076655, PubMed:33268465, PubMed:34518685, PubMed:38418882). Acts as an atypical E3 ubiquitin-protein ligase by working together with cullin-5-RING ubiquitin ligase complex (ECS complex, also named CRL5 complex) and initiating ubiquitination of ECS substrates: associates with ECS complex and specifically mediates addition of the first ubiquitin on ECS targets (PubMed:33268465, PubMed:34518685, PubMed:38418882). The initial ubiquitin is then elongated (PubMed:33268465). E3 ubiquitin-protein ligase activity is activated upon binding to neddylated form of the cullin-5 (CUL5) component of the ECS complex (PubMed:24076655). Together with the ECS(ASB9) complex, catalyzes ubiquitination of CKB (PubMed:33268465). Promotes ubiquitination of DCUN1D1 (PubMed:30587576). Mediates 'Lys-6', 'Lys-48'- and 'Lys-63'-linked polyubiquitination (PubMed:16118314, PubMed:17646546, PubMed:19340006). May play a role in myelopoiesis (PubMed:19340006)
2: (Microbial infection) Following infection by HIV-1 virus, acts together with a cullin-5-RING E3 ubiquitin-protein ligase complex (ECS complex) hijacked by the HIV-1 Vif protein, to catalyze ubiquitination and degradation of APOBEC3F and APOBEC3G