Adenylyltransferase and sulfurtransferase MOCS3 (Molybdenum cofactor synthesis protein 3) (Molybdopterin synthase sulfurylase) (MPT synthase sulfurylase) [Includes: Molybdopterin-synthase adenylyltransferase (EC 2.7.7.80) (Adenylyltransferase MOCS3) (Sulfur carrier protein MOCS2A adenylyltransferase); Molybdopterin-synthase sulfurtransferase (EC 2.8.1.11) (Sulfur carrier protein MOCS2A sulfurtransferase) (Sulfurtransferase MOCS3)]
1_MASRE 6_ EVLAL 11_ QAEVA 16_ QREEE 21_ LNSLK 26_ QKLAS 31_ ALLAE 36_ QEPQP 41_ ERLVP 46_ VSPLP 51_ PKAAL 56_ SRDEI 61_ LRYSR 66_ QLVLP 71_ ELGVH 76_ GQLRL 81_ GTACV 86_ LIVGC 91_ GGLGC 96_ PLAQY 101_ LAAAG 106_ VGRLG 111_ LVDYD 116_ VVEMS 121_ NLARQ 126_ VLHGE 131_ ALAGQ 136_ AKAFS 141_ AAASL 146_ RRLNS 151_ AVECV 156_ PYTQA 161_ LTPAT 166_ ALDLV 171_ RRYDV 176_ VADCS 181_ DNVPT 186_ RYLVN 191_ DACVL 196_ AGRPL 201_ VSASA 206_ LRFEG 211_ QITVY 216_ HYDGG 221_ PCYRC 226_ IFPQP 231_ PPAET 236_ VTNCA 241_ DGGVL 246_ GVVTG 251_ VLGCL 256_ QALEV 261_ LKIAA 266_ GLGPS 271_ YSGSL 276_ LLFDA 281_ LRGHF 286_ RSIRL 291_ RSRRL 296_ DCAAC 301_ GERPT 306_ VTDLL 311_ DYEAF 316_ CGSSA 321_ TDKCR 326_ SLQLL 331_ SPEER 336_ VSVTD 341_ YKRLL 346_ DSGAF 351_ HLLLD 356_ VRPQV 361_ EVDIC 366_ RLPHA 371_ LHIPL 376_ KHLER 381_ RDAES 386_ LKLLK 391_ EAIWE 396_ EKQGT 401_ QEGAA 406_ VPIYV 411_ ICKLG 416_ NDSQK 421_ AVKIL 426_ QSLSA 431_ AQELD 436_ PLTVR 441_ DVVGG 446_ LMAWA 451_AKIDG
1: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln) (PubMed:19017811, PubMed:22453920, PubMed:30817134). Also essential during biosynthesis of the molybdenum cofactor (PubMed:15073332, PubMed:22453920, PubMed:30817134). Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A (PubMed:15073332, PubMed:19017811, PubMed:22453920). Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus (PubMed:19017811, PubMed:22453920). The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as a nucleophile towards URM1 and MOCS2A (PubMed:15073332, PubMed:22453920). Subsequently, a transient disulfide bond is formed (PubMed:15073332, PubMed:22453920). Does not use thiosulfate as sulfur donor; NFS1 acting as a sulfur donor for thiocarboxylation reactions (PubMed:18650437, PubMed:22453920)