Serine/threonine-protein kinase OSR1 (EC 2.7.11.1) (Oxidative stress-responsive 1 protein)
1_MSEDS 6_ SALPW 11_ SINRD 16_ DYELQ 21_ EVIGS 26_ GATAV 31_ VQAAY 36_ CAPKK 41_ EKVAI 46_ KRINL 51_ EKCQT 56_ SMDEL 61_ LKEIQ 66_ AMSQC 71_ HHPNI 76_ VSYYT 81_ SFVVK 86_ DELWL 91_ VMKLL 96_ SGGSV 101_ LDIIK 106_ HIVAK 111_ GEHKS 116_ GVLDE 121_ STIAT 126_ ILREV 131_ LEGLE 136_ YLHKN 141_ GQIHR 146_ DVKAG 151_ NILLG 156_ EDGSV 161_ QIADF 166_ GVSAF 171_ LATGG 176_ DITRN 181_ KVRKT 186_ FVGTP 191_ CWMAP 196_ EVMEQ 201_ VRGYD 206_ FKADI 211_ WSFGI 216_ TAIEL 221_ ATGAA 226_ PYHKY 231_ PPMKV 236_ LMLTL 241_ QNDPP 246_ SLETG 251_ VQDKE 256_ MLKKY 261_ GKSFR 266_ KMISL 271_ CLQKD 276_ PEKRP 281_ TAAEL 286_ LRHKF 291_ FQKAK 296_ NKEFL 301_ QEKTL 306_ QRAPT 311_ ISERA 316_ KKVRR 321_ VPGSS 326_ GRLHK 331_ TEDGG 336_ WEWSD 341_ DEFDE 346_ ESEEG 351_ KAAIS 356_ QLRSP 361_ RVKES 366_ ISNSE 371_ LFPTT 376_ DPVGT 381_ LLQVP 386_ EQISA 391_ HLPQP 396_ AGQIA 401_ TQPTQ 406_ VSLPP 411_ TAEPA 416_ KTAQA 421_ LSSGS 426_ GSQET 431_ KIPIS 436_ LVLRL 441_ RNSKK 446_ ELNDI 451_ RFEFT 456_ PGRDT 461_ AEGVS 466_ QELIS 471_ AGLVD 476_ GRDLV 481_ IVAAN 486_ LQKIV 491_ EEPQS 496_ NRSVT 501_ FKLAS 506_ GVEGS 511_ DIPDD 516_ GKLIG 521_FAQLS
1: Effector serine/threonine-protein kinase component of the WNK-SPAK/OSR1 kinase cascade, which is involved in various processes, such as ion transport, response to hypertonic stress and blood pressure (PubMed:16669787, PubMed:18270262, PubMed:21321328, PubMed:34289367). Specifically recognizes and binds proteins with a RFXV motif (PubMed:16669787, PubMed:17721439, PubMed:21321328). Acts downstream of WNK kinases (WNK1, WNK2, WNK3 or WNK4): following activation by WNK kinases, catalyzes phosphorylation of ion cotransporters, such as SLC12A1/NKCC2, SLC12A2/NKCC1, SLC12A3/NCC, SLC12A5/KCC2 or SLC12A6/KCC3, regulating their activity (PubMed:17721439). Mediates regulatory volume increase in response to hyperosmotic stress by catalyzing phosphorylation of ion cotransporters SLC12A1/NKCC2, SLC12A2/NKCC1 and SLC12A6/KCC3 downstream of WNK1 and WNK3 kinases (PubMed:16669787, PubMed:21321328). Phosphorylation of Na-K-Cl cotransporters SLC12A2/NKCC1 and SLC12A2/NKCC1 promote their activation and ion influx; simultaneously, phosphorylation of K-Cl cotransporters SLC12A5/KCC2 and SLC12A6/KCC3 inhibit their activity, blocking ion efflux (PubMed:16669787, PubMed:19665974, PubMed:21321328). Acts as a regulator of NaCl reabsorption in the distal nephron by mediating phosphorylation and activation of the thiazide-sensitive Na-Cl cotransporter SLC12A3/NCC in distal convoluted tubule cells of kidney downstream of WNK4 (PubMed:18270262). Also acts as a regulator of angiogenesis in endothelial cells downstream of WNK1 (PubMed:23386621, PubMed:25362046). Acts as an activator of inward rectifier potassium channels KCNJ2/Kir2.1 and KCNJ4/Kir2.3 downstream of WNK1: recognizes and binds the RXFXV/I variant motif on KCNJ2/Kir2.1 and KCNJ4/Kir2.3 and regulates their localization to the cell membrane without mediating their phosphorylation (PubMed:29581290). Phosphorylates RELL1, RELL2 and RELT (PubMed:16389068, PubMed:28688764). Phosphorylates PAK1 (PubMed:14707132). Phosphorylates PLSCR1 in the presence of RELT (PubMed:22052202)