Apoptosis-inducing factor 1, mitochondrial (EC 1.6.99.-) (Programmed cell death protein 8)
1_MFRCG 6_ GLAAG 11_ ALKQK 16_ LVPLV 21_ RTVCV 26_ RSPRQ 31_ RNRLP 36_ GNLFQ 41_ RWHVP 46_ LELQM 51_ TRQMA 56_ SSGAS 61_ GGKID 66_ NSVLV 71_ LIVGL 76_ STVGA 81_ GAYAY 86_ KTMKE 91_ DEKRY 96_ NERIS 101_ GLGLT 106_ PEQKQ 111_ KKAAL 116_ SASEG 121_ EEVPQ 126_ DKAPS 131_ HVPFL 136_ LIGGG 141_ TAAFA 146_ AARSI 151_ RARDP 156_ GARVL 161_ IVSED 166_ PELPY 171_ MRPPL 176_ SKELW 181_ FSDDP 186_ NVTKT 191_ LRFKQ 196_ WNGKE 201_ RSIYF 206_ QPPSF 211_ YVSAQ 216_ DLPHI 221_ ENGGV 226_ AVLTG 231_ KKVVQ 236_ LDVRD 241_ NMVKL 246_ NDGSQ 251_ ITYEK 256_ CLIAT 261_ GGTPR 266_ SLSAI 271_ DRAGA 276_ EVKSR 281_ TTLFR 286_ KIGDF 291_ RSLEK 296_ ISREV 301_ KSITI 306_ IGGGF 311_ LGSEL 316_ ACALG 321_ RKARA 326_ LGTEV 331_ IQLFP 336_ EKGNM 341_ GKILP 346_ EYLSN 351_ WTMEK 356_ VRREG 361_ VKVMP 366_ NAIVQ 371_ SVGVS 376_ SGKLL 381_ IKLKD 386_ GRKVE 391_ TDHIV 396_ AAVGL 401_ EPNVE 406_ LAKTG 411_ GLEID 416_ SDFGG 421_ FRVNA 426_ ELQAR 431_ SNIWV 436_ AGDAA 441_ CFYDI 446_ KLGRR 451_ RVEHH 456_ DHAVV 461_ SGRLA 466_ GENMT 471_ GAAKP 476_ YWHQS 481_ MFWSD 486_ LGPDV 491_ GYEAI 496_ GLVDS 501_ SLPTV 506_ GVFAK 511_ ATAQD 516_ NPKSA 521_ TEQSG 526_ TGIRS 531_ ESETE 536_ SEASE 541_ ITIPP 546_ STPAV 551_ PQAPV 556_ QGEDY 561_ GKGVI 566_ FYLRD 571_ KVVVG 576_ IVLWN 581_ IFNRM 586_ PIARK 591_ IIKDG 596_ EQHED 601_ LNEVA 606_KLFNI
1: Functions both as NADH oxidoreductase and as regulator of apoptosis (PubMed:17094969, PubMed:20362274, PubMed:23217327, PubMed:33168626). In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway (PubMed:20362274). Release into the cytoplasm is mediated upon binding to poly-ADP-ribose chains (By similarity). The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA (PubMed:20362274). Binds to DNA in a sequence-independent manner (PubMed:27178839). Interacts with EIF3G, and thereby inhibits the EIF3 machinery and protein synthesis, and activates caspase-7 to amplify apoptosis (PubMed:17094969). Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells (PubMed:19418225). In contrast, participates in normal mitochondrial metabolism. Plays an important role in the regulation of respiratory chain biogenesis by interacting with CHCHD4 and controlling CHCHD4 mitochondrial import (PubMed:26004228)
2: Has NADH oxidoreductase activity. Does not induce nuclear apoptosis
3: Pro-apoptotic isoform