DNA topoisomerase 3-beta-1 (EC 5.6.2.1) (DNA topoisomerase III beta-1)
1_MKTVL 6_ MVAEK 11_ PSLAQ 16_ SIAKI 21_ LSRGS 26_ LSSHK 31_ GLNGA 36_ CSVHE 41_ YTGTF 46_ AGQPV 51_ RFKMT 56_ SVCGH 61_ VMTLD 66_ FLGKY 71_ NKWDK 76_ VDPAE 81_ LFSQA 86_ PTEKK 91_ EANPK 96_ LNMVK 101_ FLQVE 106_ GRGCD 111_ YIVLW 116_ LDCDK 121_ EGENI 126_ CFEVL 131_ DAVLP 136_ VMNKA 141_ HGGEK 146_ TVFRA 151_ RFSSI 156_ TDTDI 161_ CNAMA 166_ CLGEP 171_ DHNEA 176_ LSVDA 181_ RQELD 186_ LRIGC 191_ AFTRF 196_ QTKYF 201_ QGKYG 206_ DLDSS 211_ LISFG 216_ PCQTP 221_ TLGFC 226_ VERHD 231_ KIQSF 236_ KPETY 241_ WVLQA 246_ KVNTD 251_ KDRSL 256_ LLDWD 261_ RVRVF 266_ DREIA 271_ QMFLN 276_ MTKLE 281_ KEAQV 286_ EATSR 291_ KEKAK 296_ QRPLA 301_ LNTVE 306_ MLRVA 311_ SSSLG 316_ MGPQH 321_ AMQTA 326_ ERLYT 331_ QGYIS 336_ YPRTE 341_ TTHYP 346_ ENFDL 351_ KGSLR 356_ QQANH 361_ PYWAD 366_ TVKRL 371_ LAEGI 376_ NRPRK 381_ GHDAG 386_ DHPPI 391_ TPMKS 396_ ATEAE 401_ LGGDA 406_ WRLYE 411_ YITRH 416_ FIATV 421_ SHDCK 426_ YLQST 431_ ISFRI 436_ GPELF 441_ TCSGK 446_ TVLSP 451_ GFTEV 456_ MPWQS 461_ VPLEE 466_ SLPTC 471_ QRGDA 476_ FPVGE 481_ VKMLE 486_ KQTNP 491_ PDYLT 496_ EAELI 501_ TLMEK 506_ HGIGT 511_ DASIP 516_ VHINN 521_ ICQRN 526_ YVTVE 531_ SGRRL 536_ KPTNL 541_ GIVLV 546_ HGYYK 551_ IDAEL 556_ VLPTI 561_ RSAVE 566_ KQLNL 571_ IAQGK 576_ ADYRQ 581_ VLGHT 586_ LDVFK 591_ RKFHY 596_ FVDSI 601_ AGMDE 606_ LMEVS 611_ FSPLA 616_ ATGKP 621_ LSRCG 626_ KCHRF 631_ MKYIQ 636_ AKPSR 641_ LHCSH 646_ CDETY 651_ TLPQN 656_ GTIKL 661_ YKELR 666_ CPLDD 671_ FELVL 676_ WSSGS 681_ RGKSY 686_ PLCPY 691_ CYNHP 696_ PFRDM 701_ KKGMG 706_ CNECT 711_ HPSCQ 716_ HSLSM 721_ LGIGQ 726_ CVECE 731_ SGVLV 736_ LDPTS 741_ GPKWK 746_ VACNK 751_ CNVVA 756_ HCFEN 761_ AHRVR 766_ VSADT 771_ CSVCE 776_ AALLD 781_ VDFNK 786_ AKSPL 791_ PGDET 796_ QHMGC 801_ VFCDP 806_ VFQEL 811_ VELKH 816_ AASCH 821_ PMHRG 826_ GPGRR 831_ QGRGR 836_ GRARR 841_ PPGKP 846_ NPRRP 851_ KDKMS 856_ALAAY
1: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Possesses negatively supercoiled DNA relaxing activity