Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (EC 1.2.1.12) (Peptidyl-cysteine S-nitrosylase GAPDH) (EC 2.6.99.-)
1_MGKVK 6_ VGVNG 11_ FGRIG 16_ RLVTR 21_ AAFNS 26_ GKVDI 31_ VAIND 36_ PFIDL 41_ NYMVY 46_ MFQYD 51_ STHGK 56_ FHGTV 61_ KAENG 66_ KLVIN 71_ GNPIT 76_ IFQER 81_ DPSKI 86_ KWGDA 91_ GAEYV 96_ VESTG 101_ VFTTM 106_ EKAGA 111_ HLQGG 116_ AKRVI 121_ ISAPS 126_ ADAPM 131_ FVMGV 136_ NHEKY 141_ DNSLK 146_ IISNA 151_ SCTTN 156_ CLAPL 161_ AKVIH 166_ DNFGI 171_ VEGLM 176_ TTVHA 181_ ITATQ 186_ KTVDG 191_ PSGKL 196_ WRDGR 201_ GALQN 206_ IIPAS 211_ TGAAK 216_ AVGKV 221_ IPELN 226_ GKLTG 231_ MAFRV 236_ PTANV 241_ SVVDL 246_ TCRLE 251_ KPAKY 256_ DDIKK 261_ VVKQA 266_ SEGPL 271_ KGILG 276_ YTEHQ 281_ VVSSD 286_ FNSDT 291_ HSSTF 296_ DAGAG 301_ IALND 306_ HFVKL 311_ ISWYD 316_ NEFGY 321_ SNRVV 326_DLMAH
1: Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively (PubMed:11724794, PubMed:3170585). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate (PubMed:11724794, PubMed:3170585). Modulates the organization and assembly of the cytoskeleton (By similarity). Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes (PubMed:23071094). Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation (PubMed:23071094). Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively (PubMed:23332158, PubMed:27387501). Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis (By similarity). Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity)