Cytochrome P450 1A2 (EC 1.14.14.1) (CYPIA2) (Cholesterol 25-hydroxylase) (Cytochrome P(3)450) (Cytochrome P450 4) (Cytochrome P450-P3) (Hydroperoxy icosatetraenoate dehydratase) (EC 4.2.1.152)
1_MALSQ 6_ SVPFS 11_ ATELL 16_ LASAI 21_ FCLVF 26_ WVLKG 31_ LRPRV 36_ PKGLK 41_ SPPEP 46_ WGWPL 51_ LGHVL 56_ TLGKN 61_ PHLAL 66_ SRMSQ 71_ RYGDV 76_ LQIRI 81_ GSTPV 86_ LVLSR 91_ LDTIR 96_ QALVR 101_ QGDDF 106_ KGRPD 111_ LYTST 116_ LITDG 121_ QSLTF 126_ STDSG 131_ PVWAA 136_ RRRLA 141_ QNALN 146_ TFSIA 151_ SDPAS 156_ SSSCY 161_ LEEHV 166_ SKEAK 171_ ALISR 176_ LQELM 181_ AGPGH 186_ FDPYN 191_ QVVVS 196_ VANVI 201_ GAMCF 206_ GQHFP 211_ ESSDE 216_ MLSLV 221_ KNTHE 226_ FVETA 231_ SSGNP 236_ LDFFP 241_ ILRYL 246_ PNPAL 251_ QRFKA 256_ FNQRF 261_ LWFLQ 266_ KTVQE 271_ HYQDF 276_ DKNSV 281_ RDITG 286_ ALFKH 291_ SKKGP 296_ RASGN 301_ LIPQE 306_ KIVNL 311_ VNDIF 316_ GAGFD 321_ TVTTA 326_ ISWSL 331_ MYLVT 336_ KPEIQ 341_ RKIQK 346_ ELDTV 351_ IGRER 356_ RPRLS 361_ DRPQL 366_ PYLEA 371_ FILET 376_ FRHSS 381_ FLPFT 386_ IPHST 391_ TRDTT 396_ LNGFY 401_ IPKKC 406_ CVFVN 411_ QWQVN 416_ HDPEL 421_ WEDPS 426_ EFRPE 431_ RFLTA 436_ DGTAI 441_ NKPLS 446_ EKMML 451_ FGMGK 456_ RRCIG 461_ EVLAK 466_ WEIFL 471_ FLAIL 476_ LQQLE 481_ FSVPP 486_ GVKVD 491_ LTPIY 496_ GLTMK 501_ HARCE 506_ HVQAR 511_LRFSI
1: A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins (PubMed:10681376, PubMed:11555828, PubMed:12865317, PubMed:19965576, PubMed:9435160). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) (PubMed:10681376, PubMed:11555828, PubMed:12865317, PubMed:19965576, PubMed:9435160). Catalyzes the hydroxylation of carbon-hydrogen bonds (PubMed:11555828, PubMed:12865317). Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2-hydroxy E1 and E2 (PubMed:11555828, PubMed:12865317). Metabolizes cholesterol toward 25-hydroxycholesterol, a physiological regulator of cellular cholesterol homeostasis (PubMed:21576599). May act as a major enzyme for all-trans retinoic acid biosynthesis in the liver. Catalyzes two successive oxidative transformation of all-trans retinol to all-trans retinal and then to the active form all-trans retinoic acid (PubMed:10681376). Primarily catalyzes stereoselective epoxidation of the last double bond of polyunsaturated fatty acids (PUFA), displaying a strong preference for the (R,S) stereoisomer (PubMed:19965576). Catalyzes bisallylic hydroxylation and omega-1 hydroxylation of PUFA (PubMed:9435160). May also participate in eicosanoids metabolism by converting hydroperoxide species into oxo metabolites (lipoxygenase-like reaction, NADPH-independent) (PubMed:21068195). Plays a role in the oxidative metabolism of xenobiotics. Catalyzes the N-hydroxylation of heterocyclic amines and the O-deethylation of phenacetin (PubMed:14725854). Metabolizes caffeine via N3-demethylation (Probable)