Bifunctional glutamate/proline--tRNA ligase (Bifunctional aminoacyl-tRNA synthetase) (Cell proliferation-inducing gene 32 protein) (Glutamatyl-prolyl-tRNA synthetase) [Includes: Glutamate--tRNA ligase (EC 6.1.1.17) (Glutamyl-tRNA synthetase) (GluRS); Proline--tRNA ligase (EC 6.1.1.15) (Prolyl-tRNA synthetase)]
1_MATLS 6_ LTVNS 11_ GDPPL 16_ GALLA 21_ VEHVK 26_ DDVSI 31_ SVEEG 36_ KENIL 41_ HVSEN 46_ VIFTD 51_ VNSIL 56_ RYLAR 61_ VATTA 66_ GLYGS 71_ NLMEH 76_ TEIDH 81_ WLEFS 86_ ATKLS 91_ SCDSF 96_ TSTIN 101_ ELNHC 106_ LSLRT 111_ YLVGN 116_ SLSLA 121_ DLCVW 126_ ATLKG 131_ NAAWQ 136_ EQLKQ 141_ KKAPV 146_ HVKRW 151_ FGFLE 156_ AQQAF 161_ QSVGT 166_ KWDVS 171_ TTKAR 176_ VAPEK 181_ KQDVG 186_ KFVEL 191_ PGAEM 196_ GKVTV 201_ RFPPE 206_ ASGYL 211_ HIGHA 216_ KAALL 221_ NQHYQ 226_ VNFKG 231_ KLIMR 236_ FDDTN 241_ PEKEK 246_ EDFEK 251_ VILED 256_ VAMLH 261_ IKPDQ 266_ FTYTS 271_ DHFET 276_ IMKYA 281_ EKLIQ 286_ EGKAY 291_ VDDTP 296_ AEQMK 301_ AEREQ 306_ RIDSK 311_ HRKNP 316_ IEKNL 321_ QMWEE 326_ MKKGS 331_ QFGQS 336_ CCLRA 341_ KIDMS 346_ SNNGC 351_ MRDPT 356_ LYRCK 361_ IQPHP 366_ RTGNK 371_ YNVYP 376_ TYDFA 381_ CPIVD 386_ SIEGV 391_ THALR 396_ TTEYH 401_ DRDEQ 406_ FYWII 411_ EALGI 416_ RKPYI 421_ WEYSR 426_ LNLNN 431_ TVLSK 436_ RKLTW 441_ FVNEG 446_ LVDGW 451_ DDPRF 456_ PTVRG 461_ VLRRG 466_ MTVEG 471_ LKQFI 476_ AAQGS 481_ SRSVV 486_ NMEWD 491_ KIWAF 496_ NKKVI 501_ DPVAP 506_ RYVAL 511_ LKKEV 516_ IPVNV 521_ PEAQE 526_ EMKEV 531_ AKHPK 536_ NPEVG 541_ LKPVW 546_ YSPKV 551_ FIEGA 556_ DAETF 561_ SEGEM 566_ VTFIN 571_ WGNLN 576_ ITKIH 581_ KNADG 586_ KIISL 591_ DAKLN 596_ LENKD 601_ YKKTT 606_ KVTWL 611_ AETTH 616_ ALPIP 621_ VICVT 626_ YEHLI 631_ TKPVL 636_ GKDED 641_ FKQYV 646_ NKNSK 651_ HEELM 656_ LGDPC 661_ LKDLK 666_ KGDII 671_ QLQRR 676_ GFFIC 681_ DQPYE 686_ PVSPY 691_ SCKEA 696_ PCVLI 701_ YIPDG 706_ HTKEM 711_ PTSGS 716_ KEKTK 721_ VEATK 726_ NETSA 731_ PFKER 736_ PTPSL 741_ NNNCT 746_ TSEDS 751_ LVLYN 756_ RVAVQ 761_ GDVVR 766_ ELKAK 771_ KAPKE 776_ DVDAA 781_ VKQLL 786_ SLKAE 791_ YKEKT 796_ GQEYK 801_ PGNPP 806_ AEIGQ 811_ NISSN 816_ SSASI 821_ LESKS 826_ LYDEV 831_ AAQGE 836_ VVRKL 841_ KAEKS 846_ PKAKI 851_ NEAVE 856_ CLLSL 861_ KAQYK 866_ EKTGK 871_ EYIPG 876_ QPPLS 881_ QSSDS 886_ SPTRN 891_ SEPAG 896_ LETPE 901_ AKVLF 906_ DKVAS 911_ QGEVV 916_ RKLKT 921_ EKAPK 926_ DQVDI 931_ AVQEL 936_ LQLKA 941_ QYKSL 946_ IGVEY 951_ KPVSA 956_ TGAED 961_ KDKKK 966_ KEKEN 971_ KSEKQ 976_ NKPQK 981_ QNDGQ 986_ RKDPS 991_ KNQGG 996_ GLSSS 1001_ GAGEG 1006_ QGPKK 1011_ QTRLG 1016_ LEAKK 1021_ EENLA 1026_ DWYSQ 1031_ VITKS 1036_ EMIEY 1041_ HDISG 1046_ CYILR 1051_ PWAYA 1056_ IWEAI 1061_ KDFFD 1066_ AEIKK 1071_ LGVEN 1076_ CYFPM 1081_ FVSQS 1086_ ALEKE 1091_ KTHVA 1096_ DFAPE 1101_ VAWVT 1106_ RSGKT 1111_ ELAEP 1116_ IAIRP 1121_ TSETV 1126_ MYPAY 1131_ AKWVQ 1136_ SHRDL 1141_ PIKLN 1146_ QWCNV 1151_ VRWEF 1156_ KHPQP 1161_ FLRTR 1166_ EFLWQ 1171_ EGHSA 1176_ FATME 1181_ EAAEE 1186_ VLQIL 1191_ DLYAQ 1196_ VYEEL 1201_ LAIPV 1206_ VKGRK 1211_ TEKEK 1216_ FAGGD 1221_ YTTTI 1226_ EAFIS 1231_ ASGRA 1236_ IQGGT 1241_ SHHLG 1246_ QNFSK 1251_ MFEIV 1256_ FEDPK 1261_ IPGEK 1266_ QFAYQ 1271_ NSWGL 1276_ TTRTI 1281_ GVMTM 1286_ VHGDN 1291_ MGLVL 1296_ PPRVA 1301_ CVQVV 1306_ IIPCG 1311_ ITNAL 1316_ SEEDK 1321_ EALIA 1326_ KCNDY 1331_ RRRLL 1336_ SVNIR 1341_ VRADL 1346_ RDNYS 1351_ PGWKF 1356_ NHWEL 1361_ KGVPI 1366_ RLEVG 1371_ PRDMK 1376_ SCQFV 1381_ AVRRD 1386_ TGEKL 1391_ TVAEN 1396_ EAETK 1401_ LQAIL 1406_ EDIQV 1411_ TLFTR 1416_ ASEDL 1421_ KTHMV 1426_ VANTM 1431_ EDFQK 1436_ ILDSG 1441_ KIVQI 1446_ PFCGE 1451_ IDCED 1456_ WIKKT 1461_ TARDQ 1466_ DLEPG 1471_ APSMG 1476_ AKSLC 1481_ IPFKP 1486_ LCELQ 1491_ PGAKC 1496_ VCGKN 1501_ PAKYY 1506_TLFGR
1: Multifunctional protein which primarily functions within the aminoacyl-tRNA synthetase multienzyme complex, also known as multisynthetase complex. Within the complex it catalyzes the attachment of both L-glutamate and L-proline to their cognate tRNAs in a two-step reaction where the amino acid is first activated by ATP to form a covalent intermediate with AMP. Subsequently, the activated amino acid is transferred to the acceptor end of the cognate tRNA to form L-glutamyl-tRNA(Glu) and L-prolyl-tRNA(Pro) (PubMed:23263184, PubMed:24100331, PubMed:29576217, PubMed:3290852, PubMed:37212275). Upon interferon-gamma stimulation, EPRS1 undergoes phosphorylation, causing its dissociation from the aminoacyl-tRNA synthetase multienzyme complex. It is recruited to form the GAIT complex, which binds to stem loop-containing GAIT elements found in the 3'-UTR of various inflammatory mRNAs, such as ceruloplasmin. The GAIT complex inhibits the translation of these mRNAs, allowing interferon-gamma to redirect the function of EPRS1 from protein synthesis to translation inhibition in specific cell contexts (PubMed:15479637, PubMed:23071094). Furthermore, it can function as a downstream effector in the mTORC1 signaling pathway, by promoting the translocation of SLC27A1 from the cytoplasm to the plasma membrane where it mediates the uptake of long-chain fatty acid by adipocytes. Thereby, EPRS1 also plays a role in fat metabolism and more indirectly influences lifespan (PubMed:28178239)