DNA polymerase alpha catalytic subunit (EC 2.7.7.7) (DNA polymerase alpha catalytic subunit p180)
1_MAPVH 6_ GDDSL 11_ SDSGS 16_ FVSSR 21_ ARREK 26_ KSKKG 31_ RQEAL 36_ ERLKK 41_ AKAGE 46_ KYKYE 51_ VEDFT 56_ GVYEE 61_ VDEEQ 66_ YSKLV 71_ QARQD 76_ DDWIV 81_ DDDGI 86_ GYVED 91_ GREIF 96_ DDDLE 101_ DDALD 106_ ADEKG 111_ KDGKA 116_ RNKDK 121_ RNVKK 126_ LAVTK 131_ PNNIK 136_ SMFIA 141_ CAGKK 146_ TADKA 151_ VDLSK 156_ DGLLG 161_ DILQD 166_ LNTET 171_ PQITP 176_ PPVMI 181_ LKKKR 186_ SIGAS 191_ PNPFS 196_ VHTAT 201_ AVPSG 206_ KIASP 211_ VSRKE 216_ PPLTP 221_ VPLKR 226_ AEFAG 231_ DDVQV 236_ ESTEE 241_ EQESG 246_ AMEFE 251_ DGDFD 256_ EPMEV 261_ EEVDL 266_ EPMAA 271_ KAWDK 276_ ESEPA 281_ EEVKQ 286_ EADSG 291_ KGTVS 296_ YLGSF 301_ LPDVS 306_ CWDID 311_ QEGDS 316_ SFSVQ 321_ EVQVD 326_ SSHLP 331_ LVKGA 336_ DEEQV 341_ FHFYW 346_ LDAYE 351_ DQYNQ 356_ PGVVF 361_ LFGKV 366_ WIESA 371_ ETHVS 376_ CCVMV 381_ KNIER 386_ TLYFL 391_ PREMK 396_ IDLNT 401_ GKETG 406_ TPISM 411_ KDVYE 416_ EFDEK 421_ IATKY 426_ KIMKF 431_ KSKPV 436_ EKNYA 441_ FEIPD 446_ VPEKS 451_ EYLEV 456_ KYSAE 461_ MPQLP 466_ QDLKG 471_ ETFSH 476_ VFGTN 481_ TSSLE 486_ LFLMN 491_ RKIKG 496_ PCWLE 501_ VKSPQ 506_ LLNQP 511_ VSWCK 516_ VEAMA 521_ LKPDL 526_ VNVIK 531_ DVSPP 536_ PLVVM 541_ AFSMK 546_ TMQNA 551_ KNHQN 556_ EIIAM 561_ AALVH 566_ HSFAL 571_ DKAAP 576_ KPPFQ 581_ SHFCV 586_ VSKPK 591_ DCIFP 596_ YAFKE 601_ VIEKK 606_ NVKVE 611_ VAATE 616_ RTLLG 621_ FFLAK 626_ VHKID 631_ PDIIV 636_ GHNIY 641_ GFELE 646_ VLLQR 651_ INVCK 656_ APHWS 661_ KIGRL 666_ KRSNM 671_ PKLGG 676_ RSGFG 681_ ERNAT 686_ CGRMI 691_ CDVEI 696_ SAKEL 701_ IRCKS 706_ YHLSE 711_ LVQQI 716_ LKTER 721_ VVIPM 726_ ENIQN 731_ MYSES 736_ SQLLY 741_ LLEHT 746_ WKDAK 751_ FILQI 756_ MCELN 761_ VLPLA 766_ LQITN 771_ IAGNI 776_ MSRTL 781_ MGGRS 786_ ERNEF 791_ LLLHA 796_ FYENN 801_ YIVPD 806_ KQIFR 811_ KPQQK 816_ LGDED 821_ EEIDG 826_ DTNKY 831_ KKGRK 836_ KAAYA 841_ GGLVL 846_ DPKVG 851_ FYDKF 856_ ILLLD 861_ FNSLY 866_ PSIIQ 871_ EFNIC 876_ FTTVQ 881_ RVASE 886_ AQKVT 891_ EDGEQ 896_ EQIPE 901_ LPDPS 906_ LEMGI 911_ LPREI 916_ RKLVE 921_ RRKQV 926_ KQLMK 931_ QQDLN 936_ PDLIL 941_ QYDIR 946_ QKALK 951_ LTANS 956_ MYGCL 961_ GFSYS 966_ RFYAK 971_ PLAAL 976_ VTYKG 981_ REILM 986_ HTKEM 991_ VQKMN 996_ LEVIY 1001_ GDTDS 1006_ IMINT 1011_ NSTNL 1016_ EEVFK 1021_ LGNKV 1026_ KSEVN 1031_ KLYKL 1036_ LEIDI 1041_ DGVFK 1046_ SLLLL 1051_ KKKKY 1056_ AALVV 1061_ EPTSD 1066_ GNYVT 1071_ KQELK 1076_ GLDIV 1081_ RRDWC 1086_ DLAKD 1091_ TGNFV 1096_ IGQIL 1101_ SDQSR 1106_ DTIVE 1111_ NIQKR 1116_ LIEIG 1121_ ENVLN 1126_ GSVPV 1131_ SQFEI 1136_ NKALT 1141_ KDPQD 1146_ YPDKK 1151_ SLPHV 1156_ HVALW 1161_ INSQG 1166_ GRKVK 1171_ AGDTV 1176_ SYVIC 1181_ QDGSN 1186_ LTASQ 1191_ RAYAP 1196_ EQLQK 1201_ QDNLT 1206_ IDTQY 1211_ YLAQQ 1216_ IHPVV 1221_ ARICE 1226_ PIDGI 1231_ DAVLI 1236_ ATWLG 1241_ LDPTQ 1246_ FRVHH 1251_ YHKDE 1256_ ENDAL 1261_ LGGPA 1266_ QLTDE 1271_ EKYRD 1276_ CERFK 1281_ CPCPT 1286_ CGTEN 1291_ IYDNV 1296_ FDGSG 1301_ TDMEP 1306_ SLYRC 1311_ SNIDC 1316_ KASPL 1321_ TFTVQ 1326_ LSNKL 1331_ IMDIR 1336_ RFIKK 1341_ YYDGW 1346_ LICEE 1351_ PTCRN 1356_ RTRHL 1361_ PLQFS 1366_ RTGPL 1371_ CPACM 1376_ KATLQ 1381_ PEYSD 1386_ KSLYT 1391_ QLCFY 1396_ RYIFD 1401_ AECAL 1406_ EKLTT 1411_ DHEKD 1416_ KLKKQ 1421_ FFTPK 1426_ VLQDY 1431_ RKLKN 1436_ TAEQF 1441_ LSRSG 1446_ YSEVN 1451_ LSKLF 1456_AGCAV
1: Catalytic subunit of the DNA polymerase alpha complex (also known as the alpha DNA polymerase-primase complex) which plays an essential role in the initiation of DNA synthesis. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1, a regulatory subunit POLA2 and two primase subunits PRIM1 and PRIM2) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes. In the cytosol, responsible for a substantial proportion of the physiological concentration of cytosolic RNA:DNA hybrids, which are necessary to prevent spontaneous activation of type I interferon responses (PubMed:27019227)