DNA topoisomerase 1 (EC 5.6.2.1) (DNA topoisomerase I)
1_MSGDH 6_ LHNDS 11_ QIEAD 16_ FRLND 21_ SHKHK 26_ DKHKD 31_ REHRH 36_ KEHKK 41_ EKDRE 46_ KSKHS 51_ NSEHK 56_ DSEKK 61_ HKEKE 66_ KTKHK 71_ DGSSE 76_ KHKDK 81_ HKDRD 86_ KEKRK 91_ EEKVR 96_ ASGDA 101_ KIKKE 106_ KENGF 111_ SSPPQ 116_ IKDEP 121_ EDDGY 126_ FVPPK 131_ EDIKP 136_ LKRPR 141_ DEDDA 146_ DYKPK 151_ KIKTE 156_ DTKKE 161_ KKRKL 166_ EEEED 171_ GKLKK 176_ PKNKD 181_ KDKKV 186_ PEPDN 191_ KKKKP 196_ KKEEE 201_ QKWKW 206_ WEEER 211_ YPEGI 216_ KWKFL 221_ EHKGP 226_ VFAPP 231_ YEPLP 236_ ENVKF 241_ YYDGK 246_ VMKLS 251_ PKAEE 256_ VATFF 261_ AKMLD 266_ HEYTT 271_ KEIFR 276_ KNFFK 281_ DWRKE 286_ MTNEE 291_ KNIIT 296_ NLSKC 301_ DFTQM 306_ SQYFK 311_ AQTEA 316_ RKQMS 321_ KEEKL 326_ KIKEE 331_ NEKLL 336_ KEYGF 341_ CIMDN 346_ HKERI 351_ ANFKI 356_ EPPGL 361_ FRGRG 366_ NHPKM 371_ GMLKR 376_ RIMPE 381_ DIIIN 386_ CSKDA 391_ KVPSP 396_ PPGHK 401_ WKEVR 406_ HDNKV 411_ TWLVS 416_ WTENI 421_ QGSIK 426_ YIMLN 431_ PSSRI 436_ KGEKD 441_ WQKYE 446_ TARRL 451_ KKCVD 456_ KIRNQ 461_ YREDW 466_ KSKEM 471_ KVRQR 476_ AVALY 481_ FIDKL 486_ ALRAG 491_ NEKEE 496_ GETAD 501_ TVGCC 506_ SLRVE 511_ HINLH 516_ PELDG 521_ QEYVV 526_ EFDFL 531_ GKDSI 536_ RYYNK 541_ VPVEK 546_ RVFKN 551_ LQLFM 556_ ENKQP 561_ EDDLF 566_ DRLNT 571_ GILNK 576_ HLQDL 581_ MEGLT 586_ AKVFR 591_ TYNAS 596_ ITLQQ 601_ QLKEL 606_ TAPDE 611_ NIPAK 616_ ILSYN 621_ RANRA 626_ VAILC 631_ NHQRA 636_ PPKTF 641_ EKSMM 646_ NLQTK 651_ IDAKK 656_ EQLAD 661_ ARRDL 666_ KSAKA 671_ DAKVM 676_ KDAKT 681_ KKVVE 686_ SKKKA 691_ VQRLE 696_ EQLMK 701_ LEVQA 706_ TDREE 711_ NKQIA 716_ LGTSK 721_ LNYLD 726_ PRITV 731_ AWCKK 736_ WGVPI 741_ EKIYN 746_ KTQRE 751_ KFAWA 756_IDMAD
1: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the BMAL1 promoter