Granzyme A (EC 3.4.21.78) (CTL tryptase) (Cytotoxic T-lymphocyte proteinase 1) (Fragmentin-1) (Granzyme-1) (Hanukkah factor) (H factor) (HF)
1_MRNSY 6_ RFLAS 11_ SLSVV 16_ VSLLL 21_ IPEDV 26_ CEKII 31_ GGNEV 36_ TPHSR 41_ PYMVL 46_ LSLDR 51_ KTICA 56_ GALIA 61_ KDWVL 66_ TAAHC 71_ NLNKR 76_ SQVIL 81_ GAHSI 86_ TREEP 91_ TKQIM 96_ LVKKE 101_ FPYPC 106_ YDPAT 111_ REGDL 116_ KLLQL 121_ MEKAK 126_ INKYV 131_ TILHL 136_ PKKGD 141_ DVKPG 146_ TMCQV 151_ AGWGR 156_ THNSA 161_ SWSDT 166_ LREVN 171_ ITIID 176_ RKVCN 181_ DRNHY 186_ NFNPV 191_ IGMNM 196_ VCAGS 201_ LRGGR 206_ DSCNG 211_ DSGSP 216_ LLCEG 221_ VFRGV 226_ TSFGL 231_ ENKCG 236_ DPRGP 241_ GVYIL 246_ LSKKH 251_ LNWII 256_MTIKG
1: Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse (PubMed:12819770, PubMed:32299851, PubMed:3257574, PubMed:3262682, PubMed:3263427). It cleaves after Lys or Arg (PubMed:12819770, PubMed:32299851). Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-B (GSDMB), releasing the pore-forming moiety of GSDMB, thereby triggering pyroptosis and target cell death (PubMed:32299851, PubMed:34022140, PubMed:36157507, PubMed:36899106). Cleaves APEX1 after 'Lys-31' and destroys its oxidative repair activity (PubMed:12524539). Cleaves the nucleosome assembly protein SET after 'Lys-189', which disrupts its nucleosome assembly activity and allows the SET complex to translocate into the nucleus to nick and degrade the DNA (PubMed:11555662, PubMed:12628186, PubMed:16818237)