X-ray repair cross-complementing protein 5 (EC 3.6.4.-) (86 kDa subunit of Ku antigen) (ATP-dependent DNA helicase 2 subunit 2) (ATP-dependent DNA helicase II 80 kDa subunit) (CTC box-binding factor 85 kDa subunit) (CTC85) (CTCBF) (DNA repair protein XRCC5) (Ku80) (Ku86) (Lupus Ku autoantigen protein p86) (Nuclear factor IV) (Thyroid-lupus autoantigen) (TLAA) (X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining))
1_MVRSG 6_ NKAAV 11_ VLCMD 16_ VGFTM 21_ SNSIP 26_ GIESP 31_ FEQAK 36_ KVITM 41_ FVQRQ 46_ VFAEN 51_ KDEIA 56_ LVLFG 61_ TDGTD 66_ NPLSG 71_ GDQYQ 76_ NITVH 81_ RHLML 86_ PDFDL 91_ LEDIE 96_ SKIQP 101_ GSQQA 106_ DFLDA 111_ LIVSM 116_ DVIQH 121_ ETIGK 126_ KFEKR 131_ HIEIF 136_ TDLSS 141_ RFSKS 146_ QLDII 151_ IHSLK 156_ KCDIS 161_ LQFFL 166_ PFSLG 171_ KEDGS 176_ GDRGD 181_ GPFRL 186_ GGHGP 191_ SFPLK 196_ GITEQ 201_ QKEGL 206_ EIVKM 211_ VMISL 216_ EGEDG 221_ LDEIY 226_ SFSES 231_ LRKLC 236_ VFKKI 241_ ERHSI 246_ HWPCR 251_ LTIGS 256_ NLSIR 261_ IAAYK 266_ SILQE 271_ RVKKT 276_ WTVVD 281_ AKTLK 286_ KEDIQ 291_ KETVY 296_ CLNDD 301_ DETEV 306_ LKEDI 311_ IQGFR 316_ YGSDI 321_ VPFSK 326_ VDEEQ 331_ MKYKS 336_ EGKCF 341_ SVLGF 346_ CKSSQ 351_ VQRRF 356_ FMGNQ 361_ VLKVF 366_ AARDD 371_ EAAAV 376_ ALSSL 381_ IHALD 386_ DLDMV 391_ AIVRY 396_ AYDKR 401_ ANPQV 406_ GVAFP 411_ HIKHN 416_ YECLV 421_ YVQLP 426_ FMEDL 431_ RQYMF 436_ SSLKN 441_ SKKYA 446_ PTEAQ 451_ LNAVD 456_ ALIDS 461_ MSLAK 466_ KDEKT 471_ DTLED 476_ LFPTT 481_ KIPNP 486_ RFQRL 491_ FQCLL 496_ HRALH 501_ PREPL 506_ PPIQQ 511_ HIWNM 516_ LNPPA 521_ EVTTK 526_ SQIPL 531_ SKIKT 536_ LFPLI 541_ EAKKK 546_ DQVTA 551_ QEIFQ 556_ DNHED 561_ GPTAK 566_ KLKTE 571_ QGGAH 576_ FSVSS 581_ LAEGS 586_ VTSVG 591_ SVNPA 596_ ENFRV 601_ LVKQK 606_ KASFE 611_ EASNQ 616_ LINHI 621_ EQFLD 626_ TNETP 631_ YFMKS 636_ IDCIR 641_ AFREE 646_ AIKFS 651_ EEQRF 656_ NNFLK 661_ ALQEK 666_ VEIKQ 671_ LNHFW 676_ EIVVQ 681_ DGITL 686_ ITKEE 691_ ASGSS 696_ VTAEE 701_ AKKFL 706_ APKDK 711_ PSGDT 716_ AAVFE 721_ EGGDV 726_DDLLD
1: Single-stranded DNA-dependent ATP-dependent helicase that plays a key role in DNA non-homologous end joining (NHEJ) by recruiting DNA-PK to DNA (PubMed:11493912, PubMed:12145306, PubMed:7957065, PubMed:8621488). Required for double-strand break repair and V(D)J recombination (PubMed:11493912, PubMed:12145306, PubMed:7957065, PubMed:8621488). Also has a role in chromosome translocation (PubMed:11493912, PubMed:12145306, PubMed:7957065, PubMed:8621488). The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner (PubMed:11493912, PubMed:12145306, PubMed:7957065, PubMed:8621488). It works in the 3'-5' direction (PubMed:11493912, PubMed:12145306, PubMed:7957065, PubMed:8621488). During NHEJ, the XRCC5-XRRC6 dimer performs the recognition step: it recognizes and binds to the broken ends of the DNA and protects them from further resection (PubMed:11493912, PubMed:12145306, PubMed:7957065, PubMed:8621488). Binding to DNA may be mediated by XRCC6 (PubMed:11493912, PubMed:12145306, PubMed:7957065, PubMed:8621488). The XRCC5-XRRC6 dimer acts as a regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold (PubMed:11493912, PubMed:12145306, PubMed:20383123, PubMed:7957065, PubMed:8621488). The XRCC5-XRRC6 dimer is probably involved in stabilizing broken DNA ends and bringing them together (PubMed:12145306, PubMed:20383123, PubMed:7957065, PubMed:8621488). The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step (PubMed:12145306, PubMed:20383123, PubMed:7957065, PubMed:8621488). The XRCC5-XRRC6 dimer probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks (PubMed:20383123). XRCC5 probably acts as the catalytic subunit of 5'-dRP activity, and allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined (PubMed:20383123). The XRCC5-XRRC6 dimer together with APEX1 acts as a negative regulator of transcription (PubMed:8621488). In association with NAA15, the XRCC5-XRRC6 dimer binds to the osteocalcin promoter and activates osteocalcin expression (PubMed:12145306). As part of the DNA-PK complex, involved in the early steps of ribosome assembly by promoting the processing of precursor rRNA into mature 18S rRNA in the small-subunit processome (PubMed:32103174). Binding to U3 small nucleolar RNA, recruits PRKDC and XRCC5/Ku86 to the small-subunit processome (PubMed:32103174). Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway (PubMed:28712728)