Replication protein A 32 kDa subunit (RP-A p32) (Replication factor A protein 2) (RF-A protein 2) (Replication protein A 34 kDa subunit) (RP-A p34)
1_MWNSG 6_ FESYG 11_ SSSYG 16_ GAGGY 21_ TQSPG 26_ GFGSP 31_ APSQA 36_ EKKSR 41_ ARAQH 46_ IVPCT 51_ ISQLL 56_ SATLV 61_ DEVFR 66_ IGNVE 71_ ISQVT 76_ IVGII 81_ RHAEK 86_ APTNI 91_ VYKID 96_ DMTAA 101_ PMDVR 106_ QWVDT 111_ DDTSS 116_ ENTVV 121_ PPETY 126_ VKVAG 131_ HLRSF 136_ QNKKS 141_ LVAFK 146_ IMPLE 151_ DMNEF 156_ TTHIL 161_ EVINA 166_ HMVLS 171_ KANSQ 176_ PSAGR 181_ APISN 186_ PGMSE 191_ AGNFG 196_ GNSFM 201_ PANGL 206_ TVAQN 211_ QVLNL 216_ IKACP 221_ RPEGL 226_ NFQDL 231_ KNQLK 236_ HMSVS 241_ SIKQA 246_ VDFLS 251_ NEGHI 256_ YSTVD 261_DDHFK
1: As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Also plays a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. RPA stimulates 5'-3' helicase activity of BRIP1/FANCJ (PubMed:17596542)