Polyunsaturated fatty acid lipoxygenase ALOX12 (EC 1.13.11.-) (Arachidonate (12S)-lipoxygenase) (12S-LOX) (12S-lipoxygenase) (EC 1.13.11.31) (Arachidonate (15S)-lipoxygenase) (EC 1.13.11.33) (Linoleate (13S)-lipoxygenase) (Lipoxin synthase 12-LO) (EC 3.3.2.-) (Platelet-type lipoxygenase 12)
1_MGRYR 6_ IRVAT 11_ GAWLF 16_ SGSYN 21_ RVQLW 26_ LVGTR 31_ GEAEL 36_ ELQLR 41_ PARGE 46_ EEEFD 51_ HDVAE 56_ DLGLL 61_ QFVRL 66_ RKHHW 71_ LVDDA 76_ WFCDR 81_ ITVQG 86_ PGACA 91_ EVAFP 96_ CYRWV 101_ QGEDI 106_ LSLPE 111_ GTARL 116_ PGDNA 121_ LDMFQ 126_ KHREK 131_ ELKDR 136_ QQIYC 141_ WATWK 146_ EGLPL 151_ TIAAD 156_ RKDDL 161_ PPNMR 166_ FHEEK 171_ RLDFE 176_ WTLKA 181_ GALEM 186_ ALKRV 191_ YTLLS 196_ SWNCL 201_ EDFDQ 206_ IFWGQ 211_ KSALA 216_ EKVRQ 221_ CWQDD 226_ ELFSY 231_ QFLNG 236_ ANPML 241_ LRRST 246_ SLPSR 251_ LVLPS 256_ GMEEL 261_ QAQLE 266_ KELQN 271_ GSLFE 276_ ADFIL 281_ LDGIP 286_ ANVIR 291_ GEKQY 296_ LAAPL 301_ VMLKM 306_ EPNGK 311_ LQPMV 316_ IQIQP 321_ PNPSS 326_ PTPTL 331_ FLPSD 336_ PPLAW 341_ LLAKS 346_ WVRNS 351_ DFQLH 356_ EIQYH 361_ LLNTH 366_ LVAEV 371_ IAVAT 376_ MRCLP 381_ GLHPI 386_ FKFLI 391_ PHIRY 396_ TMEIN 401_ TRART 406_ QLISD 411_ GGIFD 416_ KAVST 421_ GGGGH 426_ VQLLR 431_ RAAAQ 436_ LTYCS 441_ LCPPD 446_ DLADR 451_ GLLGL 456_ PGALY 461_ AHDAL 466_ RLWEI 471_ IARYV 476_ EGIVH 481_ LFYQR 486_ DDIVK 491_ GDPEL 496_ QAWCR 501_ EITEV 506_ GLCQA 511_ QDRGF 516_ PVSFQ 521_ SQSQL 526_ CHFLT 531_ MCVFT 536_ CTAQH 541_ AAINQ 546_ GQLDW 551_ YAWVP 556_ NAPCT 561_ MRMPP 566_ PTTKE 571_ DVTMA 576_ TVMGS 581_ LPDVR 586_ QACLQ 591_ MAISW 596_ HLSRR 601_ QPDMV 606_ PLGHH 611_ KEKYF 616_ SGPKP 621_ KAVLN 626_ QFRTD 631_ LEKLE 636_ KEITA 641_ RNEQL 646_ DWPYE 651_ YLKPS 656_CIENS
1: Catalyzes the regio and stereo-specific incorporation of molecular oxygen into free and esterified polyunsaturated fatty acids generating lipid hydroperoxides that can be further reduced to the corresponding hydroxy species (PubMed:17493578, PubMed:18311922, PubMed:1851637, PubMed:32404334, PubMed:8319693, PubMed:8500694). Mainly converts arachidonate ((5Z,8Z,11Z,14Z)-eicosatetraenoate) to the specific bioactive lipid (12S)-hydroperoxyeicosatetraenoate/(12S)-HPETE (PubMed:17493578, PubMed:22984144, PubMed:24282679, PubMed:8319693, PubMed:8500694). Through the production of bioactive lipids like (12S)-HPETE it regulates different biological processes including platelet activation (PubMed:8319693, PubMed:8500694). It can also catalyze the epoxidation of double bonds of polyunsaturated fatty acids such as (14S)-hydroperoxy-docosahexaenoate/(14S)-HPDHA resulting in the formation of (13S,14S)-epoxy-DHA (PubMed:23504711). Furthermore, it may participate in the sequential oxidations of DHA ((4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate) to generate specialized pro-resolving mediators (SPMs) like resolvin D5 ((7S,17S)-diHPDHA) and (7S,14S)-diHPDHA, that actively down-regulate the immune response and have anti-aggregation properties with platelets (PubMed:32404334). An additional function involves a multistep process by which it transforms leukotriene A4/LTA4 into the bioactive lipids lipoxin A4/LXA4 and lipoxin B4/LXB4, both are vasoactive and LXA4 may regulate neutrophil function via occupancy of specific recognition sites (PubMed:8250832). Can also peroxidize linoleate ((9Z,12Z)-octadecadienoate) to (13S)-hydroperoxyoctadecadienoate/ (13S-HPODE) (By similarity). Due to its role in regulating both the expression of the vascular endothelial growth factor (VEGF, an angiogenic factor involved in the survival and metastasis of solid tumors) and the expression of integrin beta-1 (known to affect tumor cell migration and proliferation), it can be regarded as protumorigenic (PubMed:16638750, PubMed:22237009, PubMed:9751607). Important for cell survival, as it may play a role not only in proliferation but also in the prevention of apoptosis in vascular smooth muscle cells (PubMed:23578768)