Peptidyl-glycine alpha-amidating monooxygenase (PAM) [Includes: Peptidylglycine alpha-hydroxylating monooxygenase (PHM) (EC 1.14.17.3); Peptidyl-alpha-hydroxyglycine alpha-amidating lyase (EC 4.3.2.5) (Peptidylamidoglycolate lyase) (PAL)]
1_MAGRV 6_ PSLLV 11_ LLVFP 16_ SSCLA 21_ FRSPL 26_ SVFKR 31_ FKETT 36_ RPFSN 41_ ECLGT 46_ TRPVV 51_ PIDSS 56_ DFALD 61_ IRMPG 66_ VTPKQ 71_ SDTYF 76_ CMSMR 81_ IPVDE 86_ EAFVI 91_ DFKPR 96_ ASMDT 101_ VHHML 106_ LFGCN 111_ MPSST 116_ GSYWF 121_ CDEGT 126_ CTDKA 131_ NILYA 136_ WARNA 141_ PPTRL 146_ PKGVG 151_ FRVGG 156_ ETGSK 161_ YFVLQ 166_ VHYGD 171_ ISAFR 176_ DNNKD 181_ CSGVS 186_ LHLTR 191_ LPQPL 196_ IAGMY 201_ LMMSV 206_ DTVIP 211_ AGEKV 216_ VNSDI 221_ SCHYK 226_ NYPMH 231_ VFAYR 236_ VHTHH 241_ LGKVV 246_ SGYRV 251_ RNGQW 256_ TLIGR 261_ QSPQL 266_ PQAFY 271_ PVGHP 276_ VDVSF 281_ GDLLA 286_ ARCVF 291_ TGEGR 296_ TEATH 301_ IGGTS 306_ SDEMC 311_ NLYIM 316_ YYMEA 321_ KHAVS 326_ FMTCT 331_ QNVAP 336_ DMFRT 341_ IPPEA 346_ NIPIP 351_ VKSDM 356_ VMMHE 361_ HHKET 366_ EYKDK 371_ IPLLQ 376_ QPKRE 381_ EEEVL 386_ DQGDF 391_ YSLLS 396_ KLLGE 401_ REDVV 406_ HVHKY 411_ NPTEK 416_ AESES 421_ DLVAE 426_ IANVV 431_ QKKDL 436_ GRSDA 441_ REGAE 446_ HERGN 451_ AILVR 456_ DRIHK 461_ FHRLV 466_ STLRP 471_ PESRV 476_ FSLQQ 481_ PPPGE 486_ GTWEP 491_ EHTGD 496_ FHMEE 501_ ALDWP 506_ GVYLL 511_ PGQVS 516_ GVALD 521_ PKNNL 526_ VIFHR 531_ GDHVW 536_ DGNSF 541_ DSKFV 546_ YQQIG 551_ LGPIE 556_ EDTIL 561_ VIDPN 566_ NAAVL 571_ QSSGK 576_ NLFYL 581_ PHGLS 586_ IDKDG 591_ NYWVT 596_ DVALH 601_ QVFKL 606_ DPNNK 611_ EGPVL 616_ ILGRS 621_ MQPGS 626_ DQNHF 631_ CQPTD 636_ VAVDP 641_ GTGAI 646_ YVSDG 651_ YCNSR 656_ IVQFS 661_ PSGKF 666_ ITQWG 671_ EESSG 676_ SSPLP 681_ GQFTV 686_ PHSLA 691_ LVPLL 696_ GQLCV 701_ ADREN 706_ GRIQC 711_ FKTDT 716_ KEFVR 721_ EIKHS 726_ SFGRN 731_ VFAIS 736_ YIPGL 741_ LFAVN 746_ GKPHF 751_ GDQEP 756_ VQGFV 761_ MNFSN 766_ GEIID 771_ IFKPV 776_ RKHFD 781_ MPHDI 786_ VASED 791_ GTVYI 796_ GDAHT 801_ NTVWK 806_ FTLTE 811_ KLEHR 816_ SVKKA 821_ GIEVQ 826_ EIKEA 831_ EAVVE 836_ TKMEN 841_ KPTSS 846_ ELQKM 851_ QEKQK 856_ LIKEP 861_ GSGVP 866_ VVLIT 871_ TLLVI 876_ PVVVL 881_ LAIAI 886_ FIRWK 891_ KSRAF 896_ GDSEH 901_ KLETS 906_ SGRVL 911_ GRFRG 916_ KGSGG 921_ LNLGN 926_ FFASR 931_ KGYSR 936_ KGFDR 941_ LSTEG 946_ SDQEK 951_ EDDGS 956_ ESEEE 961_ YSAPL 966_PALAP
1: Bifunctional enzyme that catalyzes amidation of the C-terminus of proteins (PubMed:12699694, PubMed:2357221). Alpha-amidation is present at the C-terminus of many endocrine hormones and neuropeptides and is required for their activity (PubMed:1575450). C-terminal amidation also takes place in response to protein fragmentation triggered by oxidative stress, promoting degradation of amidated protein fragments by the proteasome (PubMed:2207077). Alpha-amidation involves two sequential reactions, both of which are catalyzed by separate catalytic domains of the enzyme (PubMed:12699694). The first step, catalyzed by peptidyl alpha-hydroxylating monooxygenase (PHM) domain, is the copper-, ascorbate-, and O2- dependent stereospecific hydroxylation (with S stereochemistry) at the alpha-carbon (C-alpha) of the C-terminal glycine of the peptidylglycine substrate (PubMed:12699694). The second step, catalyzed by the peptidylglycine amidoglycolate lyase (PAL) domain, is the zinc-dependent cleavage of the N-C-alpha bond, producing the alpha-amidated peptide and glyoxylate (PubMed:12699694). Similarly, catalyzes the two-step conversion of an N-fatty acylglycine to a primary fatty acid amide and glyoxylate (By similarity)