rRNA 2'-O-methyltransferase fibrillarin (EC 2.1.1.-) (34 kDa nucleolar scleroderma antigen) (Histone-glutamine methyltransferase) (U6 snRNA 2'-O-methyltransferase fibrillarin)
1_MKPGF 6_ SPRGG 11_ GFGGR 16_ GGFGD 21_ RGGRG 26_ GRGGF 31_ GGGRG 36_ RGGGF 41_ RGRGR 46_ GGGGG 51_ GGGGG 56_ GGGRG 61_ GGGFH 66_ SGGNR 71_ GRGRG 76_ GKRGN 81_ QSGKN 86_ VMVEP 91_ HRHEG 96_ VFICR 101_ GKEDA 106_ LVTKN 111_ LVPGE 116_ SVYGE 121_ KRVSI 126_ SEGDD 131_ KIEYR 136_ AWNPF 141_ RSKLA 146_ AAILG 151_ GVDQI 156_ HIKPG 161_ AKVLY 166_ LGAAS 171_ GTTVS 176_ HVSDI 181_ VGPDG 186_ LVYAV 191_ EFSHR 196_ SGRDL 201_ INLAK 206_ KRTNI 211_ IPVIE 216_ DARHP 221_ HKYRM 226_ LIAMV 231_ DVIFA 236_ DVAQP 241_ DQTRI 246_ VALNA 251_ HTFLR 256_ NGGHF 261_ VISIK 266_ ANCID 271_ STASA 276_ EAVFA 281_ SEVKK 286_ MQQEN 291_ MKPQE 296_ QLTLE 301_ PYERD 306_ HAVVV 311_ GVYRP 316_PPKVK
1: S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins (PubMed:24352239, PubMed:30540930, PubMed:32017898). Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA (PubMed:30540930). Site specificity is provided by a guide RNA that base pairs with the substrate (By similarity). Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA (By similarity). Probably catalyzes 2'-O-methylation of U6 snRNAs in box C/D RNP complexes (PubMed:32017898). U6 snRNA 2'-O-methylation is required for mRNA splicing fidelity (PubMed:32017898). Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (PubMed:24352239, PubMed:30540930). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed:34516797)