Cytochrome P450 7A1 (24-hydroxycholesterol 7-alpha-hydroxylase) (EC 1.14.14.26) (CYPVII) (Cholesterol 7-alpha-hydroxylase) (Cholesterol 7-alpha-monooxygenase) (EC 1.14.14.23)
1_MMTTS 6_ LIWGI 11_ AIAAC 16_ CCLWL 21_ ILGIR 26_ RRQTG 31_ EPPLE 36_ NGLIP 41_ YLGCA 46_ LQFGA 51_ NPLEF 56_ LRANQ 61_ RKHGH 66_ VFTCK 71_ LMGKY 76_ VHFIT 81_ NPLSY 86_ HKVLC 91_ HGKYF 96_ DWKKF 101_ HFATS 106_ AKAFG 111_ HRSID 116_ PMDGN 121_ TTENI 126_ NDTFI 131_ KTLQG 136_ HALNS 141_ LTESM 146_ MENLQ 151_ RIMRP 156_ PVSSN 161_ SKTAA 166_ WVTEG 171_ MYSFC 176_ YRVMF 181_ EAGYL 186_ TIFGR 191_ DLTRR 196_ DTQKA 201_ HILNN 206_ LDNFK 211_ QFDKV 216_ FPALV 221_ AGLPI 226_ HMFRT 231_ AHNAR 236_ EKLAE 241_ SLRHE 246_ NLQKR 251_ ESISE 256_ LISLR 261_ MFLND 266_ TLSTF 271_ DDLEK 276_ AKTHL 281_ VVLWA 286_ SQANT 291_ IPATF 296_ WSLFQ 301_ MIRNP 306_ EAMKA 311_ ATEEV 316_ KRTLE 321_ NAGQK 326_ VSLEG 331_ NPICL 336_ SQAEL 341_ NDLPV 346_ LDSII 351_ KESLR 356_ LSSAS 361_ LNIRT 366_ AKEDF 371_ TLHLE 376_ DGSYN 381_ IRKDD 386_ IIALY 391_ PQLMH 396_ LDPEI 401_ YPDPL 406_ TFKYD 411_ RYLDE 416_ NGKTK 421_ TTFYC 426_ NGLKL 431_ KYYYM 436_ PFGSG 441_ ATICP 446_ GRLFA 451_ IHEIK 456_ QFLIL 461_ MLSYF 466_ ELELI 471_ EGQAK 476_ CPPLD 481_ QSRAG 486_ LGILP 491_ PLNDI 496_EFKYK
1: A cytochrome P450 monooxygenase involved in the metabolism of endogenous cholesterol and its oxygenated derivatives (oxysterols) (PubMed:11013305, PubMed:12077124, PubMed:19965590, PubMed:21813643, PubMed:2384150). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed:11013305, PubMed:12077124, PubMed:19965590, PubMed:21813643, PubMed:2384150). Functions as a critical regulatory enzyme of bile acid biosynthesis and cholesterol homeostasis. Catalyzes the hydroxylation of carbon hydrogen bond at 7-alpha position of cholesterol, a rate-limiting step in cholesterol catabolism and bile acid biosynthesis (PubMed:12077124, PubMed:19965590, PubMed:2384150). 7-alpha hydroxylates several oxysterols, including 4beta-hydroxycholesterol and 24-hydroxycholesterol (PubMed:11013305, PubMed:12077124). Catalyzes the oxidation of the 7,8 double bond of 7-dehydrocholesterol and lathosterol with direct and predominant formation of the 7-keto derivatives (PubMed:21813643)