DNA polymerase delta catalytic subunit (EC 2.7.7.7) (3'-5' exodeoxyribonuclease) (EC 3.1.11.-) (DNA polymerase subunit delta p125)
1_MDGKR 6_ RPGPG 11_ PGVPP 16_ KRARG 21_ GLWDD 26_ DDAPR 31_ PSQFE 36_ EDLAL 41_ MEEME 46_ AEHRL 51_ QEQEE 56_ EELQS 61_ VLEGV 66_ ADGQV 71_ PPSAI 76_ DPRWL 81_ RPTPP 86_ ALDPQ 91_ TEPLI 96_ FQQLE 101_ IDHYV 106_ GPAQP 111_ VPGGP 116_ PPSRG 121_ SVPVL 126_ RAFGV 131_ TDEGF 136_ SVCCH 141_ IHGFA 146_ PYFYT 151_ PAPPG 156_ FGPEH 161_ MGDLQ 166_ RELNL 171_ AISRD 176_ SRGGR 181_ ELTGP 186_ AVLAV 191_ ELCSR 196_ ESMFG 201_ YHGHG 206_ PSPFL 211_ RITVA 216_ LPRLV 221_ APARR 226_ LLEQG 231_ IRVAG 236_ LGTPS 241_ FAPYE 246_ ANVDF 251_ EIRFM 256_ VDTDI 261_ VGCNW 266_ LELPA 271_ GKYAL 276_ RLKEK 281_ ATQCQ 286_ LEADV 291_ LWSDV 296_ VSHPP 301_ EGPWQ 306_ RIAPL 311_ RVLSF 316_ DIECA 321_ GRKGI 326_ FPEPE 331_ RDPVI 336_ QICSL 341_ GLRWG 346_ EPEPF 351_ LRLAL 356_ TLRPC 361_ APILG 366_ AKVQS 371_ YEKEE 376_ DLLQA 381_ WSTFI 386_ RIMDP 391_ DVITG 396_ YNIQN 401_ FDLPY 406_ LISRA 411_ QTLKV 416_ QTFPF 421_ LGRVA 426_ GLCSN 431_ IRDSS 436_ FQSKQ 441_ TGRRD 446_ TKVVS 451_ MVGRV 456_ QMDML 461_ QVLLR 466_ EYKLR 471_ SYTLN 476_ AVSFH 481_ FLGEQ 486_ KEDVQ 491_ HSIIT 496_ DLQNG 501_ NDQTR 506_ RRLAV 511_ YCLKD 516_ AYLPL 521_ RLLER 526_ LMVLV 531_ NAVEM 536_ ARVTG 541_ VPLSY 546_ LLSRG 551_ QQVKV 556_ VSQLL 561_ RQAMH 566_ EGLLM 571_ PVVKS 576_ EGGED 581_ YTGAT 586_ VIEPL 591_ KGYYD 596_ VPIAT 601_ LDFSS 606_ LYPSI 611_ MMAHN 616_ LCYTT 621_ LLRPG 626_ TAQKL 631_ GLTED 636_ QFIRT 641_ PTGDE 646_ FVKTS 651_ VRKGL 656_ LPQIL 661_ ENLLS 666_ ARKRA 671_ KAELA 676_ KETDP 681_ LRRQV 686_ LDGRQ 691_ LALKV 696_ SANSV 701_ YGFTG 706_ AQVGK 711_ LPCLE 716_ ISQSV 721_ TGFGR 726_ QMIEK 731_ TKQLV 736_ ESKYT 741_ VENGY 746_ STSAK 751_ VVYGD 756_ TDSVM 761_ CRFGV 766_ SSVAE 771_ AMALG 776_ REAAD 781_ WVSGH 786_ FPSPI 791_ RLEFE 796_ KVYFP 801_ YLLIS 806_ KKRYA 811_ GLLFS 816_ SRPDA 821_ HDRMD 826_ CKGLE 831_ AVRRD 836_ NCPLV 841_ ANLVT 846_ ASLRR 851_ LLIDR 856_ DPEGA 861_ VAHAQ 866_ DVISD 871_ LLCNR 876_ IDISQ 881_ LVITK 886_ ELTRA 891_ ASDYA 896_ GKQAH 901_ VELAE 906_ RMRKR 911_ DPGSA 916_ PSLGD 921_ RVPYV 926_ IISAA 931_ KGVAA 936_ YMKSE 941_ DPLFV 946_ LEHSL 951_ PIDTQ 956_ YYLEQ 961_ QLAKP 966_ LLRIF 971_ EPILG 976_ EGRAE 981_ AVLLR 986_ GDHTR 991_ CKTVL 996_ TGKVG 1001_ GLLAF 1006_ AKRRN 1011_ CCIGC 1016_ RTVLS 1021_ HQGAV 1026_ CEFCQ 1031_ PRESE 1036_ LYQKE 1041_ VSHLN 1046_ ALEER 1051_ FSRLW 1056_ TQCQR 1061_ CQGSL 1066_ HEDVI 1071_ CTSRD 1076_ CPIFY 1081_ MRKKV 1086_ RKDLE 1091_ DQEQL 1096_ LRRFG 1101_PPGPE
1: As the catalytic component of the trimeric (Pol-delta3 complex) and tetrameric DNA polymerase delta complexes (Pol-delta4 complex), plays a crucial role in high fidelity genome replication, including in lagging strand synthesis, and repair (PubMed:16510448, PubMed:19074196, PubMed:20334433, PubMed:24022480, PubMed:24035200, PubMed:31449058). Exhibits both DNA polymerase and 3'- to 5'-exonuclease activities (PubMed:16510448, PubMed:19074196, PubMed:20334433, PubMed:24022480, PubMed:24035200). Requires the presence of accessory proteins POLD2, POLD3 and POLD4 for full activity. Depending upon the absence (Pol-delta3) or the presence of POLD4 (Pol-delta4), displays differences in catalytic activity. Most notably, expresses higher proofreading activity in the context of Pol-delta3 compared with that of Pol-delta4 (PubMed:19074196, PubMed:20334433). Although both Pol-delta3 and Pol-delta4 process Okazaki fragments in vitro, Pol-delta3 may be better suited to fulfill this task, exhibiting near-absence of strand displacement activity compared to Pol-delta4 and stalling on encounter with the 5'-blocking oligonucleotides. Pol-delta3 idling process may avoid the formation of a gap, while maintaining a nick that can be readily ligated (PubMed:24035200). Along with DNA polymerase kappa, DNA polymerase delta carries out approximately half of nucleotide excision repair (NER) synthesis following UV irradiation (PubMed:20227374). Under conditions of DNA replication stress, in the presence of POLD3 and POLD4, may catalyze the repair of broken replication forks through break-induced replication (BIR) (PubMed:24310611). Involved in the translesion synthesis (TLS) of templates carrying O6-methylguanine, 8oxoG or abasic sites (PubMed:19074196, PubMed:24191025)