Wee1-like protein kinase (WEE1hu) (EC 2.7.10.2) (Wee1A kinase)
1_MSFLS 6_ RQQPP 11_ PPRRA 16_ GAACT 21_ LRQKL 26_ IFSPC 31_ SDCEE 36_ EEEEE 41_ EEEGS 46_ GHSTG 51_ EDSAF 56_ QEPDS 61_ PLPPA 66_ RSPTE 71_ PGPER 76_ RRSPG 81_ PAPGS 86_ PGELE 91_ EDLLL 96_ PGACP 101_ GADEA 106_ GGGAE 111_ GDSWE 116_ EEGFG 121_ SSSPV 126_ KSPAA 131_ PYFLG 136_ SSFSP 141_ VRCGG 146_ PGDAS 151_ PRGCG 156_ ARRAG 161_ EGRRS 166_ PRPDH 171_ PGTPP 176_ HKTFR 181_ KLRLF 186_ DTPHT 191_ PKSLL 196_ SKARG 201_ IDSSS 206_ VKLRG 211_ SSLFM 216_ DTEKS 221_ GKREF 226_ DVRQT 231_ PQVNI 236_ NPFTP 241_ DSLLL 246_ HSSGQ 251_ CRRRK 256_ RTYWN 261_ DSCGE 266_ DMEAS 271_ DYELE 276_ DETRP 281_ AKRIT 286_ ITESN 291_ MKSRY 296_ TTEFH 301_ ELEKI 306_ GSGEF 311_ GSVFK 316_ CVKRL 321_ DGCIY 326_ AIKRS 331_ KKPLA 336_ GSVDE 341_ QNALR 346_ EVYAH 351_ AVLGQ 356_ HSHVV 361_ RYFSA 366_ WAEDD 371_ HMLIQ 376_ NEYCN 381_ GGSLA 386_ DAISE 391_ NYRIM 396_ SYFKE 401_ AELKD 406_ LLLQV 411_ GRGLR 416_ YIHSM 421_ SLVHM 426_ DIKPS 431_ NIFIS 436_ RTSIP 441_ NAASE 446_ EGDED 451_ DWASN 456_ KVMFK 461_ IGDLG 466_ HVTRI 471_ SSPQV 476_ EEGDS 481_ RFLAN 486_ EVLQE 491_ NYTHL 496_ PKADI 501_ FALAL 506_ TVVCA 511_ AGAEP 516_ LPRNG 521_ DQWHE 526_ IRQGR 531_ LPRIP 536_ QVLSQ 541_ EFTEL 546_ LKVMI 551_ HPDPE 556_ RRPSA 561_ MALVK 566_ HSVLL 571_ SASRK 576_ SAEQL 581_ RIELN 586_ AEKFK 591_ NSLLQ 596_ KELKK 601_ AQMAK 606_ AAAEE 611_ RALFT 616_ DRMAT 621_ RSTTQ 626_ SNRTS 631_ RLIGK 636_ KMNRS 641_VSLTI
1: Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15' (PubMed:15070733, PubMed:7743995, PubMed:8348613, PubMed:8428596). Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase (PubMed:7743995, PubMed:8348613, PubMed:8428596). Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur (PubMed:7743995, PubMed:8348613, PubMed:8428596). Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated (PubMed:7743995). A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation (PubMed:7743995)