DNA-directed RNA polymerase II subunit RPB2 (EC 2.7.7.6) (DNA-directed RNA polymerase II 140 kDa polypeptide) (DNA-directed RNA polymerase II subunit B) (RNA polymerase II subunit 2) (RNA polymerase II subunit B2)
1_MYDAD 6_ EDMQY 11_ DEDDD 16_ EITPD 21_ LWQEA 26_ CWIVI 31_ SSYFD 36_ EKGLV 41_ RQQLD 46_ SFDEF 51_ IQMSV 56_ QRIVE 61_ DAPPI 66_ DLQAE 71_ AQHAS 76_ GEVEE 81_ PPRYL 86_ LKFEQ 91_ IYLSK 96_ PTHWE 101_ RDGAP 106_ SPMMP 111_ NEARL 116_ RNLTY 121_ SAPLY 126_ VDITK 131_ TVIKE 136_ GEEQL 141_ QTQHQ 146_ KTFIG 151_ KIPIM 156_ LRSTY 161_ CLLNG 166_ LTDRD 171_ LCELN 176_ ECPLD 181_ PGGYF 186_ IINGS 191_ EKVLI 196_ AQEKM 201_ ATNTV 206_ YVFAK 211_ KDSKY 216_ AYTGE 221_ CRSCL 226_ ENSSR 231_ PTSTI 236_ WVSML 241_ ARGGQ 246_ GAKKS 251_ AIGQR 256_ IVATL 261_ PYIKQ 266_ EVPII 271_ IVFRA 276_ LGFVS 281_ DRDIL 286_ EHIIY 291_ DFEDP 296_ EMMEM 301_ VKPSL 306_ DEAFV 311_ IQEQN 316_ VALNF 321_ IGSRG 326_ AKPGV 331_ TKEKR 336_ IKYAK 341_ EVLQK 346_ EMLPH 351_ VGVSD 356_ FCETK 361_ KAYFL 366_ GYMVH 371_ RLLLA 376_ ALGRR 381_ ELDDR 386_ DHYGN 391_ KRLDL 396_ AGPLL 401_ AFLFR 406_ GMFKN 411_ LLKEV 416_ RIYAQ 421_ KFIDR 426_ GKDFN 431_ LELAI 436_ KTRII 441_ SDGLK 446_ YSLAT 451_ GNWGD 456_ QKKAH 461_ QARAG 466_ VSQVL 471_ NRLTF 476_ ASTLS 481_ HLRRL 486_ NSPIG 491_ RDGKL 496_ AKPRQ 501_ LHNTL 506_ WGMVC 511_ PAETP 516_ EGHAV 521_ GLVKN 526_ LALMA 531_ YISVG 536_ SQPSP 541_ ILEFL 546_ EEWSM 551_ ENLEE 556_ ISPAA 561_ IADAT 566_ KIFVN 571_ GCWVG 576_ IHKDP 581_ EQLMN 586_ TLRKL 591_ RRQMD 596_ IIVSE 601_ VSMIR 606_ DIRER 611_ EIRIY 616_ TDAGR 621_ ICRPL 626_ LIVEK 631_ QKLLL 636_ KKRHI 641_ DQLKE 646_ REYNN 651_ YSWQD 656_ LVASG 661_ VVEYI 666_ DTLEE 671_ ETVML 676_ AMTPD 681_ DLQEK 686_ EVAYC 691_ STYTH 696_ CEIHP 701_ SMILG 706_ VCASI 711_ IPFPD 716_ HNQSP 721_ RNTYQ 726_ SAMGK 731_ QAMGV 736_ YITNF 741_ HVRMD 746_ TLAHV 751_ LYYPQ 756_ KPLVT 761_ TRSME 766_ YLRFR 771_ ELPAG 776_ INSIV 781_ AIASY 786_ TGYNQ 791_ EDSVI 796_ MNRSA 801_ VDRGF 806_ FRSVF 811_ YRSYK 816_ EQESK 821_ KGFDQ 826_ EEVFE 831_ KPTRE 836_ TCQGM 841_ RHAIY 846_ DKLDD 851_ DGLIA 856_ PGVRV 861_ SGDDV 866_ IIGKT 871_ VTLPE 876_ NEDEL 881_ ESTNR 886_ RYTKR 891_ DCSTF 896_ LRTSE 901_ TGIVD 906_ QVMVT 911_ LNQEG 916_ YKFCK 921_ IRVRS 926_ VRIPQ 931_ IGDKF 936_ ASRHG 941_ QKGTC 946_ GIQYR 951_ QEDMP 956_ FTCEG 961_ ITPDI 966_ IINPH 971_ AIPSR 976_ MTIGH 981_ LIECL 986_ QGKVS 991_ ANKGE 996_ IGDAT 1001_ PFNDA 1006_ VNVQK 1011_ ISNLL 1016_ SDYGY 1021_ HLRGN 1026_ EVLYN 1031_ GFTGR 1036_ KITSQ 1041_ IFIGP 1046_ TYYQR 1051_ LKHMV 1056_ DDKIH 1061_ SRARG 1066_ PIQIL 1071_ NRQPM 1076_ EGRSR 1081_ DGGLR 1086_ FGEME 1091_ RDCQI 1096_ AHGAA 1101_ QFLRE 1106_ RLFEA 1111_ SDPYQ 1116_ VHVCN 1121_ LCGIM 1126_ AIANT 1131_ RTHTY 1136_ ECRGC 1141_ RNKTQ 1146_ ISLVR 1151_ MPYAC 1156_ KLLFQ 1161_ ELMSM 1166_SIAPR
1: Catalytic core component of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase which synthesizes mRNA precursors and many functional non-coding RNAs using the four ribonucleoside triphosphates as substrates (By similarity) (PubMed:27193682, PubMed:30190596, PubMed:9852112). Pol II-mediated transcription cycle proceeds through transcription initiation, transcription elongation and transcription termination stages. During transcription initiation, Pol II pre-initiation complex (PIC) is recruited to DNA promoters, with focused-type promoters containing either the initiator (Inr) element, or the TATA-box found in cell-type specific genes and dispersed-type promoters that often contain hypomethylated CpG islands usually found in housekeeping genes. Once the polymerase has escaped from the promoter it enters the elongation phase during which RNA is actively polymerized, based on complementarity with the template DNA strand. Transcription termination involves the release of the RNA transcript and polymerase from the DNA (PubMed:27193682, PubMed:30190596, PubMed:9852112). Forms Pol II active center together with the largest subunit POLR2A/RPB1. Appends one nucleotide at a time to the 3' end of the nascent RNA, with POLR2A/RPB1 most likely contributing a Mg(2+)-coordinating DxDGD motif and POLR2B/RPB2 participating in the coordination of a second Mg(2+) ion and providing lysine residues believed to facilitate Watson-Crick base pairing between the incoming nucleotide and template base. Typically, Mg(2+) ions direct a 5' nucleoside triphosphate to form a phosphodiester bond with the 3' hydroxyl of the preceding nucleotide of the nascent RNA, with the elimination of pyrophosphate. The reversible pyrophosphorolysis can occur at high pyrophosphate concentrations (By similarity) (PubMed:30190596, PubMed:9852112). Can proofread the nascent RNA transcript by means of a 3' -> 5' exonuclease activity. If a ribonucleotide is mis-incorporated, backtracks along the template DNA and cleaves the phosphodiester bond releasing the mis-incorporated 5'-ribonucleotide (By similarity) (PubMed:8381534)
2: RNA-dependent RNA polymerase that catalyzes the extension of a non-coding RNA (ncRNA) at the 3'-end using the four ribonucleoside triphosphates as substrates. An internal ncRNA sequence near the 3'-end serves as a template in a single-round Pol II-mediated RNA polymerization reaction. May decrease the stability of ncRNAs that repress Pol II-mediated gene transcription