Choline kinase alpha (CK) (EC 2.7.1.32) (CHETK-alpha) (Ethanolamine kinase) (EK) (EC 2.7.1.82)
1_MKTKF 6_ CTGGE 11_ AEPSP 16_ LGLLL 21_ SCGSG 26_ SAAPA 31_ PGVGQ 36_ QRDAA 41_ SDLES 46_ KQLGG 51_ QQPPL 56_ ALPPP 61_ PPLPL 66_ PLPLP 71_ QPPPP 76_ QPPAD 81_ EQPEP 86_ RTRRR 91_ AYLWC 96_ KEFLP 101_ GAWRG 106_ LREDE 111_ FHISV 116_ IRGGL 121_ SNMLF 126_ QCSLP 131_ DTTAT 136_ LGDEP 141_ RKVLL 146_ RLYGA 151_ ILQMR 156_ SCNKE 161_ GSEQA 166_ QKENE 171_ FQGAE 176_ AMVLE 181_ SVMFA 186_ ILAER 191_ SLGPK 196_ LYGIF 201_ PQGRL 206_ EQFIP 211_ SRRLD 216_ TEELS 221_ LPDIS 226_ AEIAE 231_ KMATF 236_ HGMKM 241_ PFNKE 246_ PKWLF 251_ GTMEK 256_ YLKEV 261_ LRIKF 266_ TEESR 271_ IKKLH 276_ KLLSY 281_ NLPLE 286_ LENLR 291_ SLLES 296_ TPSPV 301_ VFCHN 306_ DCQEG 311_ NILLL 316_ EGREN 321_ SEKQK 326_ LMLID 331_ FEYSS 336_ YNYRG 341_ FDIGN 346_ HFCEW 351_ MYDYS 356_ YEKYP 361_ FFRAN 366_ IRKYP 371_ TKKQQ 376_ LHFIS 381_ SYLPA 386_ FQNDF 391_ ENLST 396_ EEKSI 401_ IKEEM 406_ LLEVN 411_ RFALA 416_ SHFLW 421_ GLWSI 426_ VQAKI 431_ SSIEF 436_ GYMDY 441_ AQARF 446_ DAYFH 451_QKRKL
1: Plays a key role in phospholipid biosynthesis by catalyzing the phosphorylation of free choline to phosphocholine, the first step in phosphatidylcholine biosynthesis (PubMed:17007874, PubMed:19915674, PubMed:23416529, PubMed:34077757). Also phosphorylates ethanolamine, thereby contributing to phosphatidylethanolamine biosynthesis (PubMed:17007874, PubMed:19915674). Has higher activity with choline (PubMed:17007874, PubMed:19915674). May contribute to tumor cell growth (PubMed:19915674)
2: This isoform plays a key role in lipolysis of lipid droplets following glucose deprivation (PubMed:34077757). In response to glucose deprivation, phosphorylated by AMPK, promoting localization to lipid droplets (PubMed:34077757). Phosphorylation is followed by acetylation by KAT5, leading to dissociation of the homodimer into a monomer (PubMed:34077757). Monomeric CHKA isoform 1 is converted into a tyrosine-protein kinase, which phosphorylates lipid droplet structural proteins PLIN2 and PLIN3, leading to lipolysis of lipid droplets (PubMed:34077757)