Flap endonuclease 1 (FEN-1) (EC 3.1.-.-) (DNase IV) (Flap structure-specific endonuclease 1) (Maturation factor 1) (MF1) (hFEN-1)
1_MGIQG 6_ LAKLI 11_ ADVAP 16_ SAIRE 21_ NDIKS 26_ YFGRK 31_ VAIDA 36_ SMSIY 41_ QFLIA 46_ VRQGG 51_ DVLQN 56_ EEGET 61_ TSHLM 66_ GMFYR 71_ TIRMM 76_ ENGIK 81_ PVYVF 86_ DGKPP 91_ QLKSG 96_ ELAKR 101_ SERRA 106_ EAEKQ 111_ LQQAQ 116_ AAGAE 121_ QEVEK 126_ FTKRL 131_ VKVTK 136_ QHNDE 141_ CKHLL 146_ SLMGI 151_ PYLDA 156_ PSEAE 161_ ASCAA 166_ LVKAG 171_ KVYAA 176_ ATEDM 181_ DCLTF 186_ GSPVL 191_ MRHLT 196_ ASEAK 201_ KLPIQ 206_ EFHLS 211_ RILQE 216_ LGLNQ 221_ EQFVD 226_ LCILL 231_ GSDYC 236_ ESIRG 241_ IGPKR 246_ AVDLI 251_ QKHKS 256_ IEEIV 261_ RRLDP 266_ NKYPV 271_ PENWL 276_ HKEAH 281_ QLFLE 286_ PEVLD 291_ PESVE 296_ LKWSE 301_ PNEEE 306_ LIKFM 311_ CGEKQ 316_ FSEER 321_ IRSGV 326_ KRLSK 331_ SRQGS 336_ TQGRL 341_ DDFFK 346_ VTGSL 351_ SSAKR 356_ KEPEP 361_ KGSTK 366_ KKAKT 371_GAAGK
1: Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA