DNA polymerase delta subunit 2 (DNA polymerase delta subunit p50)
1_MFSEQ 6_ AAQRA 11_ HTLLS 16_ PPSAN 21_ NATFA 26_ RVPVA 31_ TYTNS 36_ SQPFR 41_ LGERS 46_ FSRQY 51_ AHIYA 56_ TRLIQ 61_ MRPFL 66_ ENRAQ 71_ QHWGS 76_ GVGVK 81_ KLCEL 86_ QPEEK 91_ CCVVG 96_ TLFKA 101_ MPLQP 106_ SILRE 111_ VSEEH 116_ NLLPQ 121_ PPRSK 126_ YIHPD 131_ DELVL 136_ EDELQ 141_ RIKLK 146_ GTIDV 151_ SKLVT 156_ GTVLA 161_ VFGSV 166_ RDDGK 171_ FLVED 176_ YCFAD 181_ LAPQK 186_ PAPPL 191_ DTDRF 196_ VLLVS 201_ GLGLG 206_ GGGGE 211_ SLLGT 216_ QLLVD 221_ VVTGQ 226_ LGDEG 231_ EQCSA 236_ AHVSR 241_ VILAG 246_ NLLSH 251_ STQSR 256_ DSINK 261_ AKYLT 266_ KKTQA 271_ ASVEA 276_ VKMLD 281_ EILLQ 286_ LSASV 291_ PVDVM 296_ PGEFD 301_ PTNYT 306_ LPQQP 311_ LHPCM 316_ FPLAT 321_ AYSTL 326_ QLVTN 331_ PYQAT 336_ IDGVR 341_ FLGTS 346_ GQNVS 351_ DIFRY 356_ SSMED 361_ HLEIL 366_ EWTLR 371_ VRHIS 376_ PTAPD 381_ TLGCY 386_ PFYKT 391_ DPFIF 396_ PECPH 401_ VYFCG 406_ NTPSF 411_ GSKII 416_ RGPED 421_ QTVLL 426_ VTVPD 431_ FSATQ 436_ TACLV 441_ NLRSL 446_ ACQPI 451_ SFSGF 456_ GAEDD 461_DLGGL
1: Accessory component of both the DNA polymerase delta complex and the DNA polymerase zeta complex (PubMed:17317665, PubMed:22801543, PubMed:24449906). As a component of the trimeric and tetrameric DNA polymerase delta complexes (Pol-delta3 and Pol-delta4, respectively), plays a role in high fidelity genome replication, including in lagging strand synthesis, and repair (PubMed:12403614, PubMed:16510448, PubMed:19074196, PubMed:20334433, PubMed:24035200). Pol-delta3 and Pol-delta4 are characterized by the absence or the presence of POLD4. They exhibit differences in catalytic activity. Most notably, Pol-delta3 shows higher proofreading activity than Pol-delta4 (PubMed:19074196, PubMed:20334433). Although both Pol-delta3 and Pol-delta4 process Okazaki fragments in vitro, Pol-delta3 may also be better suited to fulfill this task, exhibiting near-absence of strand displacement activity compared to Pol-delta4 and stalling on encounter with the 5'-blocking oligonucleotides. Pol-delta3 idling process may avoid the formation of a gap, while maintaining a nick that can be readily ligated (PubMed:24035200). Along with DNA polymerase kappa, DNA polymerase delta carries out approximately half of nucleotide excision repair (NER) synthesis following UV irradiation (PubMed:20227374). Under conditions of DNA replication stress, required for the repair of broken replication forks through break-induced replication (BIR) (PubMed:24310611). Involved in the translesion synthesis (TLS) of templates carrying O6-methylguanine or abasic sites performed by Pol-delta4, independently of DNA polymerase zeta (REV3L) or eta (POLH). Facilitates abasic site bypass by DNA polymerase delta by promoting extension from the nucleotide inserted opposite the lesion. Also involved in TLS as a component of the DNA polymerase zeta complex (PubMed:24449906). Along with POLD3, dramatically increases the efficiency and processivity of DNA synthesis of the DNA polymerase zeta complex compared to the minimal zeta complex, consisting of only REV3L and REV7 (PubMed:24449906)