MAP kinase-activated protein kinase 2 (MAPK-activated protein kinase 2) (MAPKAP kinase 2) (MAPKAP-K2) (MAPKAPK-2) (MK-2) (MK2) (EC 2.7.11.1)
1_MLSNS 6_ QGQSP 11_ PVPFP 16_ APAPP 21_ PQPPT 26_ PALPH 31_ PPAQP 36_ PPPPP 41_ QQFPQ 46_ FHVKS 51_ GLQIK 56_ KNAII 61_ DDYKV 66_ TSQVL 71_ GLGIN 76_ GKVLQ 81_ IFNKR 86_ TQEKF 91_ ALKML 96_ QDCPK 101_ ARREV 106_ ELHWR 111_ ASQCP 116_ HIVRI 121_ VDVYE 126_ NLYAG 131_ RKCLL 136_ IVMEC 141_ LDGGE 146_ LFSRI 151_ QDRGD 156_ QAFTE 161_ REASE 166_ IMKSI 171_ GEAIQ 176_ YLHSI 181_ NIAHR 186_ DVKPE 191_ NLLYT 196_ SKRPN 201_ AILKL 206_ TDFGF 211_ AKETT 216_ SHNSL 221_ TTPCY 226_ TPYYV 231_ APEVL 236_ GPEKY 241_ DKSCD 246_ MWSLG 251_ VIMYI 256_ LLCGY 261_ PPFYS 266_ NHGLA 271_ ISPGM 276_ KTRIR 281_ MGQYE 286_ FPNPE 291_ WSEVS 296_ EEVKM 301_ LIRNL 306_ LKTEP 311_ TQRMT 316_ ITEFM 321_ NHPWI 326_ MQSTK 331_ VPQTP 336_ LHTSR 341_ VLKED 346_ KERWE 351_ DVKEE 356_ MTSAL 361_ ATMRV 366_ DYEQI 371_ KIKKI 376_ EDASN 381_ PLLLK 386_ RRKKA 391_RALEA
1: Stress-activated serine/threonine-protein kinase involved in cytokine production, endocytosis, reorganization of the cytoskeleton, cell migration, cell cycle control, chromatin remodeling, DNA damage response and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue. Phosphorylates ALOX5, CDC25B, CDC25C, CEP131, ELAVL1, HNRNPA0, HSP27/HSPB1, KRT18, KRT20, LIMK1, LSP1, PABPC1, PARN, PDE4A, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36. Phosphorylates HSF1; leading to the interaction with HSP90 proteins and inhibiting HSF1 homotrimerization, DNA-binding and transactivation activities (PubMed:16278218). Mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to the dissociation of HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impairment of their chaperone activities and ability to protect against oxidative stress effectively. Involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally: acts by phosphorylating AU-rich elements (AREs)-binding proteins ELAVL1, HNRNPA0, PABPC1 and TTP/ZFP36, leading to the regulation of the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity, leading to inhibition of dependent degradation of ARE-containing transcripts. Phosphorylates CEP131 in response to cellular stress induced by ultraviolet irradiation which promotes binding of CEP131 to 14-3-3 proteins and inhibits formation of novel centriolar satellites (PubMed:26616734). Also involved in late G2/M checkpoint following DNA damage through a process of post-transcriptional mRNA stabilization: following DNA damage, relocalizes from nucleus to cytoplasm and phosphorylates HNRNPA0 and PARN, leading to stabilization of GADD45A mRNA. Involved in toll-like receptor signaling pathway (TLR) in dendritic cells: required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3