DNA primase small subunit (EC 2.7.7.102) (DNA primase 49 kDa subunit) (p49)
1_METFD 6_ PTELP 11_ ELLKL 16_ YYRRL 21_ FPYSQ 26_ YYRWL 31_ NYGGV 36_ IKNYF 41_ QHREF 46_ SFTLK 51_ DDIYI 56_ RYQSF 61_ NNQSD 66_ LEKEM 71_ QKMNP 76_ YKIDI 81_ GAVYS 86_ HRPNQ 91_ HNTVK 96_ LGAFQ 101_ AQEKE 106_ LVFDI 111_ DMTDY 116_ DDVRR 121_ CCSSA 126_ DICPK 131_ CWTLM 136_ TMAIR 141_ IIDRA 146_ LKEDF 151_ GFKHR 156_ LWVYS 161_ GRRGV 166_ HCWVC 171_ DESVR 176_ KLSSA 181_ VRSGI 186_ VEYLS 191_ LVKGG 196_ QDVKK 201_ KVHLS 206_ EKIHP 211_ FIRKS 216_ INIIK 221_ KYFEE 226_ YALVN 231_ QDILE 236_ NKESW 241_ DKILA 246_ LVPET 251_ IHDEL 256_ QQSFQ 261_ KSHNS 266_ LQRWE 271_ HLKKV 276_ ASRYQ 281_ NNIKN 286_ DKYGP 291_ WLEWE 296_ IMLQY 301_ CFPRL 306_ DINVS 311_ KGINH 316_ LLKSP 321_ FSVHP 326_ KTGRI 331_ SVPID 336_ LQKVD 341_ QFDPF 346_ TVPTI 351_ SFICR 356_ ELDAI 361_ STNEE 366_ EKEEN 371_ EAESD 376_ VKHRT 381_ RDYKK 386_ TSLAP 391_ YVKVF 396_ EHFLE 401_ NLDKS 406_ RKGEL 411_LKKSD
1: Catalytic subunit of the DNA primase complex and component of the DNA polymerase alpha complex (also known as the alpha DNA polymerase-primase complex - primosome/replisome) which play an essential role in the initiation of DNA synthesis (PubMed:17893144, PubMed:24043831, PubMed:25550159, PubMed:26975377, PubMed:31479243, PubMed:33060134, PubMed:9268648, PubMed:9705292). During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1, an accessory subunit POLA2 and two primase subunits, the catalytic subunit PRIM1 and the regulatory subunit PRIM2) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1 (By similarity). The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands (PubMed:17893144). These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively (By similarity). In the primase complex, both subunits are necessary for the initial di-nucleotide formation, but the extension of the primer depends only on the catalytic subunit (PubMed:17893144). Synthesizes 9-mer RNA primers (also known as the 'unit length' RNA primers). Incorporates only ribonucleotides in the presence of ribo- and deoxy-nucleotide triphosphates (rNTPs, dNTPs) (PubMed:26975377). Requires template thymine or cytidine to start the RNA primer synthesis, with an adenine or guanine at its 5'-end (PubMed:25550159, PubMed:26975377). Binds single stranded DNA (By similarity)