DNA primase large subunit (DNA primase 58 kDa subunit) (p58)
1_MEFSG 6_ RKWRK 11_ LRLAG 16_ DQRNA 21_ SYPHC 26_ LQFYL 31_ QPPSE 36_ NISLI 41_ EFENL 46_ AIDRV 51_ KLLKS 56_ VENLG 61_ VSYVK 66_ GTEQY 71_ QSKLE 76_ SELRK 81_ LKFSY 86_ RENLE 91_ DEYEP 96_ RRRDH 101_ ISHFI 106_ LRLAY 111_ CQSEE 116_ LRRWF 121_ IQQEM 126_ DLLRF 131_ RFSIL 136_ PKDKI 141_ QDFLK 146_ DSQLQ 151_ FEAIS 156_ DEEKT 161_ LREQE 166_ IVASS 171_ PSLSG 176_ LKLGF 181_ ESIYK 186_ IPFAD 191_ ALDLF 196_ RGRKV 201_ YLEDG 206_ FAYVP 211_ LKDIV 216_ AIILN 221_ EFRAK 226_ LSKAL 231_ ALTAR 236_ SLPAV 241_ QSDER 246_ LQPLL 251_ NHLSH 256_ SYTGQ 261_ DYSTQ 266_ GNVGK 271_ ISLDQ 276_ IDLLS 281_ TKSFP 286_ PCMRQ 291_ LHKAL 296_ RENHH 301_ LRHGG 306_ RMQYG 311_ LFLKG 316_ IGLTL 321_ EQALQ 326_ FWKQE 331_ FIKGK 336_ MDPDK 341_ FDKGY 346_ SYNIR 351_ HSFGK 356_ EGKRT 361_ DYTPF 366_ SCLKI 371_ ILSNP 376_ PSQGD 381_ YHGCP 386_ FRHSD 391_ PELLK 396_ QKLQS 401_ YKISP 406_ GGISQ 411_ ILDLV 416_ KGTHY 421_ QVACQ 426_ KYFEM 431_ IHNVD 436_ DCGFS 441_ LNHPN 446_ QFFCE 451_ SQRIL 456_ NGGKD 461_ IKKEP 466_ IQPET 471_ PQPKP 476_ SVQKT 481_ KDASS 486_ ALASL 491_ NSSLE 496_ MDMEG 501_LEDYF
1: Regulatory subunit of the DNA primase complex and component of the DNA polymerase alpha complex (also known as the alpha DNA polymerase-primase complex) which play an essential role in the initiation of DNA synthesis (PubMed:17893144, PubMed:25550159, PubMed:26975377, PubMed:9705292). During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1, an accessory subunit POLA2 and two primase subunits, the catalytic subunit PRIM1 and the regulatory subunit PRIM2) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1 (By similarity). The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands (PubMed:17893144). These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively (By similarity). In the primase complex, both subunits are necessary for the initial di-nucleotide formation, but the extension of the primer depends only on the catalytic subunit (PubMed:17893144, PubMed:25550159). Binds RNA:DNA duplex and coordinates the catalytic activities of PRIM1 and POLA2 during primase-to-polymerase switch