DNA ligase 4 (EC 6.5.1.1) (DNA ligase IV) (Polydeoxyribonucleotide synthase [ATP] 4)
1_MAASQ 6_ TSQTV 11_ ASHVP 16_ FADLC 21_ STLER 26_ IQKSK 31_ GRAEK 36_ IRHFR 41_ EFLDS 46_ WRKFH 51_ DALHK 56_ NHKDV 61_ TDSFY 66_ PAMRL 71_ ILPQL 76_ ERERM 81_ AYGIK 86_ ETMLA 91_ KLYIE 96_ LLNLP 101_ RDGKD 106_ ALKLL 111_ NYRTP 116_ TGTHG 121_ DAGDF 126_ AMIAY 131_ FVLKP 136_ RCLQK 141_ GSLTI 146_ QQVND 151_ LLDSI 156_ ASNNS 161_ AKRKD 166_ LIKKS 171_ LLQLI 176_ TQSSA 181_ LEQKW 186_ LIRMI 191_ IKDLK 196_ LGVSQ 201_ QTIFS 206_ VFHND 211_ AAELH 216_ NVTTD 221_ LEKVC 226_ RQLHD 231_ PSVGL 236_ SDISI 241_ TLFSA 246_ FKPML 251_ AAIAD 256_ IEHIE 261_ KDMKH 266_ QSFYI 271_ ETKLD 276_ GERMQ 281_ MHKDG 286_ DVYKY 291_ FSRNG 296_ YNYTD 301_ QFGAS 306_ PTEGS 311_ LTPFI 316_ HNAFK 321_ ADIQI 326_ CILDG 331_ EMMAY 336_ NPNTQ 341_ TFMQK 346_ GTKFD 351_ IKRMV 356_ EDSDL 361_ QTCYC 366_ VFDVL 371_ MVNNK 376_ KLGHE 381_ TLRKR 386_ YEILS 391_ SIFTP 396_ IPGRI 401_ EIVQK 406_ TQAHT 411_ KNEVI 416_ DALNE 421_ AIDKR 426_ EEGIM 431_ VKQPL 436_ SIYKP 441_ DKRGE 446_ GWLKI 451_ KPEYV 456_ SGLMD 461_ ELDIL 466_ IVGGY 471_ WGKGS 476_ RGGMM 481_ SHFLC 486_ AVAEK 491_ PPPGE 496_ KPSVF 501_ HTLSR 506_ VGSGC 511_ TMKEL 516_ YDLGL 521_ KLAKY 526_ WKPFH 531_ RKAPP 536_ SSILC 541_ GTEKP 546_ EVYIE 551_ PCNSV 556_ IVQIK 561_ AAEIV 566_ PSDMY 571_ KTGCT 576_ LRFPR 581_ IEKIR 586_ DDKEW 591_ HECMT 596_ LDDLE 601_ QLRGK 606_ ASGKL 611_ ASKHL 616_ YIGGD 621_ DEPQE 626_ KKRKA 631_ APKMK 636_ KVIGI 641_ IEHLK 646_ APNLT 651_ NVNKI 656_ SNIFE 661_ DVEFC 666_ VMSGT 671_ DSQPK 676_ PDLEN 681_ RIAEF 686_ GGYIV 691_ QNPGP 696_ DTYCV 701_ IAGSE 706_ NIRVK 711_ NIILS 716_ NKHDV 721_ VKPAW 726_ LLECF 731_ KTKSF 736_ VPWQP 741_ RFMIH 746_ MCPST 751_ KEHFA 756_ REYDC 761_ YGDSY 766_ FIDTD 771_ LNQLK 776_ EVFSG 781_ IKNSN 786_ EQTPE 791_ EMASL 796_ IADLE 801_ YRYSW 806_ DCSPL 811_ SMFRR 816_ HTVYL 821_ DSYAV 826_ INDLS 831_ TKNEG 836_ TRLAI 841_ KALEL 846_ RFHGA 851_ KVVSC 856_ LAEGV 861_ SHVII 866_ GEDHS 871_ RVADF 876_ KAFRR 881_ TFKRK 886_ FKILK 891_ ESWVT 896_ DSIDK 901_ CELQE 906_ENQYL
1: DNA ligase involved in DNA non-homologous end joining (NHEJ); required for double-strand break (DSB) repair and V(D)J recombination (PubMed:12517771, PubMed:17290226, PubMed:23523427, PubMed:29980672, PubMed:33586762, PubMed:8798671, PubMed:9242410, PubMed:9809069). Catalyzes the NHEJ ligation step of the broken DNA during DSB repair by resealing the DNA breaks after the gap filling is completed (PubMed:12517771, PubMed:17290226, PubMed:9242410, PubMed:9809069). Joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction (PubMed:12517771, PubMed:17290226, PubMed:9242410, PubMed:9809069). LIG4 is mechanistically flexible: it can ligate nicks as well as compatible DNA overhangs alone, while in the presence of XRCC4, it can ligate ends with 2-nucleotides (nt) microhomology and 1-nt gaps (PubMed:17290226). Forms a subcomplex with XRCC4; the LIG4-XRCC4 subcomplex is responsible for the NHEJ ligation step and XRCC4 enhances the joining activity of LIG4 (PubMed:9242410, PubMed:9809069). Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends (PubMed:10854421). LIG4 regulates nuclear localization of XRCC4 (PubMed:24984242)