RNA-binding protein Nova-1 (Neuro-oncological ventral antigen 1) (Onconeural ventral antigen 1) (Paraneoplastic Ri antigen) (Ventral neuron-specific protein 1)
1_MMAAA 6_ PIQQN 11_ GTHTG 16_ VPIDL 21_ DPPDS 26_ RKRPL 31_ EAPPE 36_ AGSTK 41_ RTNTG 46_ EDGQY 51_ FLKVL 56_ IPSYA 61_ AGSII 66_ GKGGQ 71_ TIVQL 76_ QKETG 81_ ATIKL 86_ SKSKD 91_ FYPGT 96_ TERVC 101_ LIQGT 106_ VEALN 111_ AVHGF 116_ IAEKI 121_ REMPQ 126_ NVAKT 131_ EPVSI 136_ LQPQT 141_ TVNPD 146_ RIKQT 151_ LPSSP 156_ TTTKS 161_ SPSDP 166_ MTTSR 171_ ANQVK 176_ IIVPN 181_ STAGL 186_ IIGKG 191_ GATVK 196_ AVMEQ 201_ SGAWV 206_ QLSQK 211_ PDGIN 216_ LQERV 221_ VTVSG 226_ EPEQN 231_ RKAVE 236_ LIIQK 241_ IQEDP 246_ QSGSC 251_ LNISY 256_ ANVTG 261_ PVANS 266_ NPTGS 271_ PYANT 276_ AEVLP 281_ TAAAA 286_ AGLLG 291_ HANLA 296_ GVAAF 301_ PAVLS 306_ GFTGN 311_ DLVAI 316_ TSALN 321_ TLASY 326_ GYNLN 331_ TLGLG 336_ LSQAA 341_ ATGAL 346_ AAAAA 351_ SANPA 356_ AAAAN 361_ LLATY 366_ ASEAS 371_ ASGST 376_ AGGTA 381_ GTFAL 386_ GSLAA 391_ ATAAT 396_ NGYFG 401_ AASPL 406_ AASAI 411_ LGTEK 416_ STDGS 421_ KDVVE 426_ IAVPE 431_ NLVGA 436_ ILGKG 441_ GKTLV 446_ EYQEL 451_ TGARI 456_ QISKK 461_ GEFVP 466_ GTRNR 471_ KVTIT 476_ GTPAA 481_ TQAAQ 486_ YLITQ 491_ RITYE 496_ QGVRA 501_ANPQK
1: Functions to regulate alternative splicing in neurons by binding pre-mRNA in a sequence-specific manner to activate exon inclusion or exclusion. It binds specifically to the sequences 5'-YCAY-3' and regulates splicing in only a subset of regulated exons (PubMed:10811881). Binding to an exonic 5'-YCAY-3' cluster changes the protein complexes assembled on pre-mRNA, blocking U1 snRNP binding and exon inclusion, whereas binding to an intronic 5'-YCAY-3' cluster enhances spliceosome assembly and exon inclusion. Binding to 5'-YCAY-3' clusters results in a local and asymmetric action to regulate spliceosome assembly and alternative splicing in neurons. Binding to an exonic 5'-YCAY-3' cluster changed the protein complexes assembled on pre-mRNA, blocking U1 snRNP (small nuclear ribonucleoprotein) binding and exon inclusion, whereas binding to an intronic 5'-YCAY-3' cluster enhanced spliceosome assembly and exon inclusion. With NOVA1, they perform unique biological functions in different brain areas and cell types. Autoregulates its own expression by acting as a splicing repressor. Acts to activate the inclusion of exon E3A in the glycine receptor alpha-2 chain and of exon E9 in gamma-aminobutyric-acid receptor gamma-2 subunit via a distal downstream UCAU-rich intronic splicing enhancer. Acts to regulate a novel glycine receptor alpha-2 chain splice variant (alpha-2N) in developing spinal cord (By similarity)