Serine/threonine-protein kinase Nek2 (EC 2.7.11.1) (HSPK 21) (Never in mitosis A-related kinase 2) (NimA-related protein kinase 2) (NimA-like protein kinase 1)
1_MPSRA 6_ EDYEV 11_ LYTIG 16_ TGSYG 21_ RCQKI 26_ RRKSD 31_ GKILV 36_ WKELD 41_ YGSMT 46_ EAEKQ 51_ MLVSE 56_ VNLLR 61_ ELKHP 66_ NIVRY 71_ YDRII 76_ DRTNT 81_ TLYIV 86_ MEYCE 91_ GGDLA 96_ SVITK 101_ GTKER 106_ QYLDE 111_ EFVLR 116_ VMTQL 121_ TLALK 126_ ECHRR 131_ SDGGH 136_ TVLHR 141_ DLKPA 146_ NVFLD 151_ GKQNV 156_ KLGDF 161_ GLARI 166_ LNHDT 171_ SFAKT 176_ FVGTP 181_ YYMSP 186_ EQMNR 191_ MSYNE 196_ KSDIW 201_ SLGCL 206_ LYELC 211_ ALMPP 216_ FTAFS 221_ QKELA 226_ GKIRE 231_ GKFRR 236_ IPYRY 241_ SDELN 246_ EIITR 251_ MLNLK 256_ DYHRP 261_ SVEEI 266_ LENPL 271_ IADLV 276_ ADEQR 281_ RNLER 286_ RGRQL 291_ GEPEK 296_ SQDSS 301_ PVLSE 306_ LKLKE 311_ IQLQE 316_ RERAL 321_ KAREE 326_ RLEQK 331_ EQELC 336_ VRERL 341_ AEDKL 346_ ARAEN 351_ LLKNY 356_ SLLKE 361_ RKFLS 366_ LASNP 371_ ELLNL 376_ PSSVI 381_ KKKVH 386_ FSGES 391_ KENIM 396_ RSENS 401_ ESQLT 406_ SKSKC 411_ KDLKK 416_ RLHAA 421_ QLRAQ 426_ ALSDI 431_ EKNYQ 436_LKSRQ
1: Protein kinase which is involved in the control of centrosome separation and bipolar spindle formation in mitotic cells and chromatin condensation in meiotic cells. Regulates centrosome separation (essential for the formation of bipolar spindles and high-fidelity chromosome separation) by phosphorylating centrosomal proteins such as CROCC, CEP250 and NINL, resulting in their displacement from the centrosomes. Regulates kinetochore microtubule attachment stability in mitosis via phosphorylation of NDC80. Involved in regulation of mitotic checkpoint protein complex via phosphorylation of CDC20 and MAD2L1. Plays an active role in chromatin condensation during the first meiotic division through phosphorylation of HMGA2. Phosphorylates: PPP1CC; SGO1; NECAB3 and NPM1. Essential for localization of MAD2L1 to kinetochore and MAPK1 and NPM1 to the centrosome. Phosphorylates CEP68 and CNTLN directly or indirectly (PubMed:24554434). NEK2-mediated phosphorylation of CEP68 promotes CEP68 dissociation from the centrosome and its degradation at the onset of mitosis (PubMed:25704143). Involved in the regulation of centrosome disjunction (PubMed:26220856). Phosphorylates CCDC102B either directly or indirectly which causes CCDC102B to dissociate from the centrosome and allows for centrosome separation (PubMed:30404835)
2: Phosphorylates and activates NEK11 in G1/S-arrested cells
3: Not present in the nucleolus and, in contrast to isoform 1, does not phosphorylate and activate NEK11 in G1/S-arrested cells