Rap1 GTPase-GDP dissociation stimulator 1 (Exchange factor smgGDS) (SMG GDS protein) (SMG P21 stimulatory GDP/GTP exchange protein)
1_MDNLS 6_ DTLKK 11_ LKITA 16_ VDKTE 21_ DSLEG 26_ CLDCL 31_ LQALA 36_ QNNTE 41_ TSEKI 46_ QASGI 51_ LQLFA 56_ SLLTP 61_ QSSCK 66_ AKVAN 71_ IIAEV 76_ AKNEF 81_ MRIPC 86_ VDAGL 91_ ISPLV 96_ QLLNS 101_ KDQEV 106_ LLQTG 111_ RALGN 116_ ICYDS 121_ HEGRS 126_ AVDQA 131_ GGAQI 136_ VIDHL 141_ RSLCS 146_ ITDPA 151_ NEKLL 156_ TVFCG 161_ MLMNY 166_ SNEND 171_ SLQAQ 176_ LINMG 181_ VIPTL 186_ VKLLG 191_ IHCQN 196_ AALTE 201_ MCLVA 206_ FGNLA 211_ ELESS 216_ KEQFA 221_ STNIA 226_ EELVK 231_ LFKKQ 236_ IEHDK 241_ REMIF 246_ EVLAP 251_ LAEND 256_ AIKLQ 261_ LVEAG 266_ LVECL 271_ LEIVQ 276_ QKVDS 281_ DKEDD 286_ ITELK 291_ TGSDL 296_ MVLLL 301_ LGDES 306_ MQKLF 311_ EGGKG 316_ SVFQR 321_ VLSWI 326_ PSNNH 331_ QLQLA 336_ GALAI 341_ ANFAR 346_ NDANC 351_ IHMVD 356_ NGIVE 361_ KLMDL 366_ LDRHV 371_ EDGNV 376_ TVQHA 381_ ALSAL 386_ RNLAI 391_ PVINK 396_ AKMLS 401_ AGVTE 406_ AVLKF 411_ LKSEM 416_ PPVQF 421_ KLLGT 426_ LRMLI 431_ DAQAE 436_ AAEQL 441_ GKNVK 446_ LVERL 451_ VEWCE 456_ AKDHA 461_ GVMGE 466_ SNRLL 471_ SALIR 476_ HSKSK 481_ DVIKT 486_ IVQSG 491_ GIKHL 496_ VTMAT 501_ SEHVI 506_ MQNEA 511_ LVALA 516_ LIAAL 521_ ELGTA 526_ EKDLE 531_ SAKLV 536_ QILHR 541_ LLADE 546_ RSAPE 551_ IKYNS 556_ MVLIC 561_ ALMGS 566_ ECLHK 571_ EVQDL 576_ AFLDV 581_ VSKLR 586_ SHENK 591_ SVAQQ 596_ ASLTE 601_QRLTV
1: Acts as a GEF (guanine nucleotide exchange factor) for the Rho family of small GTP-binding proteins (G proteins) that stimulates the dissociation of GDP to enable subsequent binding of GTP (PubMed:11948427, PubMed:1549351, PubMed:20709748, PubMed:28630045, PubMed:30190425). Additionally, appears to chaperone the processing and/or trafficking of small GTPases containing a C-terminal polybasic region independently of GEF activity (PubMed:20709748, PubMed:21242305). Targets include RAP1A/RAP1B, RHOA, RHOB, RHOC, RAC1 and KRAS (PubMed:11948427, PubMed:1549351, PubMed:20709748, PubMed:24415755). Regulates mitochondrial dynamics by controlling RHOT function to promote mitochondrial fission during high calcium conditions (PubMed:27716788). Able to promote the Ca(2+) release from the endoplasmic reticulum via both inositol trisphosphate (Ins3P) and ryanodine sensitive receptors leading to a enhanced mitochondrial Ca(2+) uptake (PubMed:24349085)
2: Acts as a GEF (guanine nucleotide exchange factor) for unprenylated RHOA (PubMed:24415755, PubMed:28630045, PubMed:30190425). Chaperones the entry and passage of small GTPases through the prenylation pathway (PubMed:20709748). Recognizes the last amino acid in the GTPase C-terminal CAAX motif with a preference for 'Leu' over 'Met', indicating involvement in the geranylgeranylation pathway (PubMed:24415755)
3: Acts as a GEF (guanine nucleotide exchange factor) for prenylated RHOA (PubMed:21242305, PubMed:28630045, PubMed:30190425). Acts as a GEF for RHOC (PubMed:21242305). Chaperones the downstream trafficking and/or processing of small newly prenylated GTPases (PubMed:20709748). Escorts RAC1 to the nucleus (PubMed:12551911)