Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1 (Glucosaminyl N-deacetylase/N-sulfotransferase 1) (NDST-1) (N-heparan sulfate sulfotransferase 1) (N-HSST 1) ([Heparan sulfate]-glucosamine N-sulfotransferase 1) (HSNST 1) [Includes: Heparan sulfate N-deacetylase 1 (EC 3.5.1.-); Heparan sulfate N-sulfotransferase 1 (EC 2.8.2.8)]
1_MPALA 6_ CLRRL 11_ CRHVS 16_ PQAVL 21_ FLLFI 26_ FCLFS 31_ VFISA 36_ YYLYG 41_ WKRGL 46_ EPSAD 51_ APEPD 56_ CGDPP 61_ PVAPS 66_ RLLPL 71_ KPVQA 76_ ATPSR 81_ TDPLV 86_ LVFVE 91_ SLYSQ 96_ LGQEV 101_ VAILE 106_ SSRFK 111_ YRTEI 116_ APGKG 121_ DMPTL 126_ TDKGR 131_ GRFAL 136_ IIYEN 141_ ILKYV 146_ NLDAW 151_ NRELL 156_ DKYCV 161_ AYGVG 166_ IIGFF 171_ KANEN 176_ SLLSA 181_ QLKGF 186_ PLFLH 191_ SNLGL 196_ KDCSI 201_ NPKSP 206_ LLYVT 211_ RPSEV 216_ EKGVL 221_ PGEDW 226_ TVFQS 231_ NHSTY 236_ EPVLL 241_ AKTRS 246_ SESIP 251_ HLGAD 256_ AGLHA 261_ ALHAT 266_ VVQDL 271_ GLHDG 276_ IQRVL 281_ FGNNL 286_ NFWLH 291_ KLVFV 296_ DAVAF 301_ LTGKR 306_ LSLPL 311_ DRYIL 316_ VDIDD 321_ IFVGK 326_ EGTRM 331_ KVEDV 336_ KALFD 341_ TQNEL 346_ RAHIP 351_ NFTFN 356_ LGYSG 361_ KFFHT 366_ GTNAE 371_ DAGDD 376_ LLLSY 381_ VKEFW 386_ WFPHM 391_ WSHMQ 396_ PHLFH 401_ NQSVL 406_ AEQMA 411_ LNKKF 416_ AVEHG 421_ IPTDM 426_ GYAVA 431_ PHHSG 436_ VYPVH 441_ VQLYE 446_ AWKQV 451_ WSIRV 456_ TSTEE 461_ YPHLK 466_ PARYR 471_ RGFIH 476_ NGIMV 481_ LPRQT 486_ CGLFT 491_ HTIFY 496_ NEYPG 501_ GSSEL 506_ DKIIN 511_ GGELF 516_ LTVLL 521_ NPISI 526_ FMTHL 531_ SNYGN 536_ DRLGL 541_ YTFKH 546_ LVRFL 551_ HSWTN 556_ LRLQT 561_ LPPVQ 566_ LAQKY 571_ FQIFS 576_ EEKDP 581_ LWQDP 586_ CEDKR 591_ HKDIW 596_ SKEKT 601_ CDRFP 606_ KLLII 611_ GPQKT 616_ GTTAL 621_ YLFLG 626_ MHPDL 631_ SSNYP 636_ SSETF 641_ EEIQF 646_ FNGHN 651_ YHKGI 656_ DWYME 661_ FFPIP 666_ SNTTS 671_ DFYFE 676_ KSANY 681_ FDSEV 686_ APRRA 691_ AALLP 696_ KAKVL 701_ TILIN 706_ PADRA 711_ YSWYQ 716_ HQRAH 721_ DDPVA 726_ LKYTF 731_ HEVIT 736_ AGSDA 741_ SSKLR 746_ ALQNR 751_ CLVPG 756_ WYATH 761_ IERWL 766_ SAYHA 771_ NQILV 776_ LDGKL 781_ LRTEP 786_ AKVMD 791_ MVQKF 796_ LGVTN 801_ TIDYH 806_ KTLAF 811_ DPKKG 816_ FWCQL 821_ LEGGK 826_ TKCLG 831_ KSKGR 836_ KYPEM 841_ DLDSR 846_ AFLKD 851_ YYRDH 856_ NIELS 861_ KLLYK 866_ MGQTL 871_ PTWLR 876_EDLQN
1: Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate (PubMed:35137078, PubMed:9230113, PubMed:9744796). Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis (PubMed:9230113). Plays a role in determining the extent and pattern of sulfation of heparan sulfate. Participates in biosynthesis of heparan sulfate that can ultimately serve as L-selectin ligands, thereby playing a role in inflammatory response (By similarity). Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413)
2: Lacks both N-deacetylase and N-sulfotransferase activities. Acts as a dominant negative on isoform 1, likely by changing the composition of enzyme complexes responsible for elongation and modification of heparan sulfates