Mismatch repair endonuclease PMS2 (EC 3.1.-.-) (DNA mismatch repair protein PMS2) (PMS1 protein homolog 2)
1_MERAE 6_ SSSTE 11_ PAKAI 16_ KPIDR 21_ KSVHQ 26_ ICSGQ 31_ VVLSL 36_ STAVK 41_ ELVEN 46_ SLDAG 51_ ATNID 56_ LKLKD 61_ YGVDL 66_ IEVSD 71_ NGCGV 76_ EEENF 81_ EGLTL 86_ KHHTS 91_ KIQEF 96_ ADLTQ 101_ VETFG 106_ FRGEA 111_ LSSLC 116_ ALSDV 121_ TISTC 126_ HASAK 131_ VGTRL 136_ MFDHN 141_ GKIIQ 146_ KTPYP 151_ RPRGT 156_ TVSVQ 161_ QLFST 166_ LPVRH 171_ KEFQR 176_ NIKKE 181_ YAKMV 186_ QVLHA 191_ YCIIS 196_ AGIRV 201_ SCTNQ 206_ LGQGK 211_ RQPVV 216_ CTGGS 221_ PSIKE 226_ NIGSV 231_ FGQKQ 236_ LQSLI 241_ PFVQL 246_ PPSDS 251_ VCEEY 256_ GLSCS 261_ DALHN 266_ LFYIS 271_ GFISQ 276_ CTHGV 281_ GRSST 286_ DRQFF 291_ FINRR 296_ PCDPA 301_ KVCRL 306_ VNEVY 311_ HMYNR 316_ HQYPF 321_ VVLNI 326_ SVDSE 331_ CVDIN 336_ VTPDK 341_ RQILL 346_ QEEKL 351_ LLAVL 356_ KTSLI 361_ GMFDS 366_ DVNKL 371_ NVSQQ 376_ PLLDV 381_ EGNLI 386_ KMHAA 391_ DLEKP 396_ MVEKQ 401_ DQSPS 406_ LRTGE 411_ EKKDV 416_ SISRL 421_ REAFS 426_ LRHTT 431_ ENKPH 436_ SPKTP 441_ EPRRS 446_ PLGQK 451_ RGMLS 456_ SSTSG 461_ AISDK 466_ GVLRP 471_ QKEAV 476_ SSSHG 481_ PSDPT 486_ DRAEV 491_ EKDSG 496_ HGSTS 501_ VDSEG 506_ FSIPD 511_ TGSHC 516_ SSEYA 521_ ASSPG 526_ DRGSQ 531_ EHVDS 536_ QEKAP 541_ KTDDS 546_ FSDVD 551_ CHSNQ 556_ EDTGC 561_ KFRVL 566_ PQPTN 571_ LATPN 576_ TKRFK 581_ KEEIL 586_ SSSDI 591_ CQKLV 596_ NTQDM 601_ SASQV 606_ DVAVK 611_ INKKV 616_ VPLDF 621_ SMSSL 626_ AKRIK 631_ QLHHE 636_ AQQSE 641_ GEQNY 646_ RKFRA 651_ KICPG 656_ ENQAA 661_ EDELR 666_ KEISK 671_ TMFAE 676_ MEIIG 681_ QFNLG 686_ FIITK 691_ LNEDI 696_ FIVDQ 701_ HATDE 706_ KYNFE 711_ MLQQH 716_ TVLQG 721_ QRLIA 726_ PQTLN 731_ LTAVN 736_ EAVLI 741_ ENLEI 746_ FRKNG 751_ FDFVI 756_ DENAP 761_ VTERA 766_ KLISL 771_ PTSKN 776_ WTFGP 781_ QDVDE 786_ LIFML 791_ SDSPG 796_ VMCRP 801_ SRVKQ 806_ MFASR 811_ ACRKS 816_ VMIGT 821_ ALNTS 826_ EMKKL 831_ ITHMG 836_ EMDHP 841_ WNCPH 846_ GRPTM 851_ RHIAN 856_LGVIS
1: Component of the post-replicative DNA mismatch repair system (MMR) (PubMed:30653781, PubMed:35189042). Heterodimerizes with MLH1 to form MutL alpha. DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Possesses an ATPase activity, but in the absence of gross structural changes, ATP hydrolysis may not be necessary for proficient mismatch repair (PubMed:35189042)