tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit WDR4 (Protein Wuho homolog) (hWH) (WD repeat-containing protein 4)
1_MAGSV 6_ GLALC 11_ GQTLV 16_ VRGGS 21_ RFLAT 26_ SIASS 31_ DDDSL 36_ FIYDC 41_ SAAEK 46_ KSQEN 51_ KGEDA 56_ PLDQG 61_ SGAIL 66_ ASTFS 71_ KSGSY 76_ FALTD 81_ DSKRL 86_ ILFRT 91_ KPWQC 96_ LSVRT 101_ VARRC 106_ TALTF 111_ IASEE 116_ KVLVA 121_ DKSGD 126_ VYSFS 131_ VLEPH 136_ GCGRL 141_ ELGHL 146_ SMLLD 151_ VAVSP 156_ DDRFI 161_ LTADR 166_ DEKIR 171_ VSWAA 176_ APHSI 181_ ESFCL 186_ GHTEF 191_ VSRIS 196_ VVPTQ 201_ PGLLL 206_ SSSGD 211_ GTLRL 216_ WEYRS 221_ GRQLH 226_ CCHLA 231_ SLQEL 236_ VDPQA 241_ PQKFA 246_ ASRIA 251_ FWCQE 256_ NCVAL 261_ LCDGT 266_ PVVYI 271_ FQLDA 276_ RRQQL 281_ VYRQQ 286_ LAFQH 291_ QVWDV 296_ AFEET 301_ QGLWV 306_ LQDCQ 311_ EAPLV 316_ LYRPV 321_ GDQWQ 326_ SVPES 331_ TVLKK 336_ VSGVL 341_ RGNWA 346_ MLEGS 351_ AGADA 356_ SFSSL 361_ YKATF 366_ DNVTS 371_ YLKKK 376_ EERLQ 381_ QQLEK 386_ KQRRR 391_ SPPPG 396_ PDGHA 401_ KKMRP 406_GEATL
1: Non-catalytic component of the METTL1-WDR4 methyltransferase complex required for the formation of N(7)-methylguanine in a subset of RNA species, such as tRNAs, mRNAs and microRNAs (miRNAs) (PubMed:12403464, PubMed:31031083, PubMed:31031084, PubMed:36599982, PubMed:36599985, PubMed:37369656). In the METTL1-WDR4 methyltransferase complex, WDR4 acts as a scaffold for tRNA-binding (PubMed:36599982, PubMed:36599985, PubMed:37369656). Required for the formation of N(7)-methylguanine at position 46 (m7G46) in a large subset of tRNAs that contain the 5'-RAGGU-3' motif within the variable loop (PubMed:12403464, PubMed:34352206, PubMed:34352207, PubMed:36599982, PubMed:36599985, PubMed:37369656). M7G46 interacts with C13-G22 in the D-loop to stabilize tRNA tertiary structure and protect tRNAs from decay (PubMed:36599982, PubMed:36599985). Also required for the formation of N(7)-methylguanine at internal sites in a subset of mRNAs (PubMed:31031084, PubMed:37379838). Also required for methylation of a specific subset of miRNAs, such as let-7 (PubMed:31031083). Independently of METTL1, also plays a role in genome stability: localizes at the DNA replication site and regulates endonucleolytic activities of FEN1 (PubMed:26751069)