Selenocysteine-specific elongation factor (Elongation factor sec) (Eukaryotic elongation factor, selenocysteine-tRNA-specific)
1_MAGRR 6_ VNVNV 11_ GVLGH 16_ IDSGK 21_ TALAR 26_ ALSTT 31_ ASTAA 36_ FDKQP 41_ QSRER 46_ GITLD 51_ LGFSC 56_ FSVPL 61_ PARLR 66_ SSLPE 71_ FQAAP 76_ EAEPE 81_ PGEPL 86_ LQVTL 91_ VDCPG 96_ HASLI 101_ RTIIG 106_ GAQII 111_ DLMML 116_ VIDVT 121_ KGMQT 126_ QSAEC 131_ LVIGQ 136_ IACQK 141_ LVVVL 146_ NKIDL 151_ LPEGK 156_ RQAAI 161_ DKMTK 166_ KMQKT 171_ LENTK 176_ FRGAP 181_ IIPVA 186_ AKPGG 191_ PEAPE 196_ TEAPQ 201_ GIPEL 206_ IELLT 211_ SQISI 216_ PTRDP 221_ SGPFL 226_ MSVDH 231_ CFSIK 236_ GQGTV 241_ MTGTI 246_ LSGSI 251_ SLGDS 256_ VEIPA 261_ LKVVK 266_ KVKSM 271_ QMFHM 276_ PITSA 281_ MQGDR 286_ LGICV 291_ TQFDP 296_ KLLER 301_ GLVCA 306_ PESLH 311_ TVHAA 316_ LISVE 321_ KIPYF 326_ RGPLQ 331_ TKAKF 336_ HITVG 341_ HETVM 346_ GRLMF 351_ FSPAP 356_ DNFDQ 361_ EPILD 366_ SFNFS 371_ QEYLF 376_ QEQYL 381_ SKDLT 386_ PAVTD 391_ NDEAD 396_ KKAGQ 401_ ATEGH 406_ CPRQQ 411_ WALVE 416_ FEKPV 421_ TCPRL 426_ CLVIG 431_ SRLDA 436_ DIHTN 441_ TCRLA 446_ FHGIL 451_ LHGLE 456_ DRNYA 461_ DSFLP 466_ RLKVY 471_ KLKHK 476_ HGLVE 481_ RAMDD 486_ YSVIG 491_ RSLFK 496_ KETNI 501_ QLFVG 506_ LKVHL 511_ STGEL 516_ GIIDS 521_ AFGQS 526_ GKFKI 531_ HIPGG 536_ LSPES 541_ KKILT 546_ PALKK 551_ RARAG 556_ RGEAT 561_ RQEES 566_ AERSE 571_ PSQHV 576_ VLSLT 581_ FKRYV 586_ FDTHK 591_RMVQS
1: Translation factor required for the incorporation of the rare amino acid selenocysteine encoded by UGA codons (PubMed:27708257, PubMed:35709277). Replaces the eRF1-eRF3-GTP ternary complex for the insertion of selenocysteine directed by the UGA codon (PubMed:27708257, PubMed:35709277). Insertion of selenocysteine at UGA codons is mediated by SECISBP2 and EEFSEC: SECISBP2 (1) specifically binds the SECIS sequence once the 80S ribosome encounters an in-frame UGA codon and (2) contacts the RPS27A/eS31 of the 40S ribosome before ribosome stalling (PubMed:35709277). (3) GTP-bound EEFSEC then delivers selenocysteinyl-tRNA(Sec) to the 80S ribosome and adopts a preaccommodated state conformation (PubMed:35709277). (4) After GTP hydrolysis, EEFSEC dissociates from the assembly, selenocysteinyl-tRNA(Sec) accommodates, and peptide bond synthesis and selenoprotein elongation occur (PubMed:35709277)